Simplified methods to isolate,culture and purify olfactory ensheathing cells

Zhengfeng Lu; Yixin Shen; Peng Zhang; Zhihai Fan; Qirong Dong; Min Wang

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Simplified methods to isolate,culture and purify olfactory ensheathing cells

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Conventional methods for harvesting,culturing and purifying olfactory ensheathing cells are complicated,time-consuming,and poorly reproducible.Olfactory bulbs were detached from adult Sprague Dawley rats and olfactory ensheathing cells were isolated using shearing,dispersion processes.After the primary cultures reached confluence,the cells were purified using a three-step process.The olfactory ensheathing cells attached and grew rapidly.The purity of the olfactory ensheathing cells increased following the three purification steps,eventually exceeding 95%.These cells could be maintained for an extended period time in culture.This simple,inexpensive,reproducible method of harvesting,culturing and purifying olfactory ensheathing cells shortens the culture cycle and provides sufficient olfactory ensheathing cells of controllable purity.

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《中国神经再生研究：英文版》 |2010年第19期|1495-1499|共5页
作者
Zhengfeng Lu; Yixin Shen; Peng Zhang; Zhihai Fan; Qirong Dong; Min Wang;
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作者单位

Department of Orthopedics;

Second Affiliated Hospital of Soochow University;

Suzhou 215004;

Jiangsu Province;

China;

Laboratory of Stem Cells;

Medical Collage;

Soochow University;

Suzhou 215004;

Jiangsu Province;

China;

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原文格式 PDF
正文语种 chi
中图分类细胞的衰老与死亡;
关键词
嗅鞘细胞; 原代培养; 细胞纯化; 分离; 可重复性; SD大鼠; 分散过程; 培养周期;

机译：嗅鞘细胞;原代培养;细胞纯化;分离;可重复性;SD大鼠;分散过程;培养周期;

Simplified methods to isolate,culture and purify olfactory ensheathing cells

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