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>A facile method for studying interaction of rhodamine B and bovine serum albumin:Towards physical-binding mediated fluorescence labeling of proteins
A facile method for studying interaction of rhodamine B and bovine serum albumin:Towards physical-binding mediated fluorescence labeling of proteins
Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein(bovine serum albumin) and a typical fluorophore(rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites.The molecular docking method has been used to study the binding site in amino acid level.
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