An X-ray microprobe for radiobiological studies was developed which deliver precise doses of radiation to the selected individual cells.The facility used synchrotron radiation as soft X-ray source.A zone plate combining with a pinhole produced a fine probe from bending magnet for single cell irradiating with defined doses.The diameter of microprobe at the target position was about 2 μm by scanning a knife-edge with an AXUV photo diode.The fluxes of soft X-rays at 516.7 eV(2.4 nm) were about 5.4×104 photons/s.100mA measured with the photo diode.The absorbed dose rate for typical yeast cells was about 11.34 Gy/s with the storage current of 100 mA.A preliminary experiment for yeast cells irradiation has shown that the microprobe had a definite biological effect for radiobiological investigations.The soft X-ray microprobe at "water window" region has provided a useful tool for single cell irradiating damage and a capability of individually irradiating a certain numbers of cells each time.
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