Preparation, characterization and radiolabelling of antigranulocytemonoclonal antibody

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Preparation, characterization and radiolabelling of antigranulocytemonoclonal antibody

机译：抗粒细胞单克隆抗体的制备，表征和放射性标记

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The human granulocytes were isolated. Using hybridoma techniques, a hybridoma cell line(HSN) producing monoclonal antibody (McAb) against human granulocyte was obtained.The antibodybelonged to IgG1 subclass. It was confirmed byimmunohistochemical tests that HSN reacted selectivelynot only with human granulocytes, but also with their bone marrow precursors. Whereas humanlymphocytesand red blood cells retained negative in the tests. No cross-reaction was observedwith theperipheral blood cells in other animals. Its affinity constant was5.7×108L/mol, and the number of epitopesper granulocyte was 4.7×105. Monoclonal antibodydisplayed no loss of immunoreactivity after labelledwith 99mTc.

机译：分离人粒细胞。利用杂交瘤技术，获得了一种抗人粒细胞单克隆抗体（McAb）的杂交瘤细胞系（HSN）。该抗体属于IgG1亚类。免疫组织化学试验证实，HSN不仅与人粒细胞选择性地反应，而且还与它们的骨髓前体反应。而人淋巴细胞和红细胞在测试中保持阴性。在其他动物中未观察到与外周血细胞的交叉反应。其亲和常数为5.7×108L / mol，表位粒细胞为4.7×105。用99mTc标记后，单克隆抗体未显示免疫反应性降低。

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《核技术（英文版）》 |1999年第4期|193-196|共4页
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Huashan Hospital, Shanghai Medical University, Shanghai 200040;

Huashan Hospital, Shanghai Medical University, Shanghai 200040;

Huashan Hospital, Shanghai Medical University, Shanghai 200040;

Huashan Hospital, Shanghai Medical University, Shanghai 200040;

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收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 chi
中图分类原子核物理学、高能物理学;
关键词
Monoclonal antibody, Human granulocytes, Technetium-99m labelling, Hybridoma;

机译：单克隆抗体;人类粒细胞;Tech 99m标记;杂交瘤;

Preparation, characterization and radiolabelling of antigranulocytemonoclonal antibody

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