Background: Protein structure in solution can be studied by small-angle X-ray scattering techniques at synchrotron radiation facilities. However, the structure of protein is prone to vary as the radiation damage induced by the intense X-ray beam during the experiments. Purpose:This study aims to develop an automatic solution-sample-changing peristaltic device for biological small angle X-ray scattering experiment. Methods: The injection pump PSD/4 of Hamilton is applied to eliminate the radiation damage of protein by minimizing the X-ray irradiation time of unit volume. In addition, automatic sample-changing, retrieving and cleaning was realized by the coordinated control of PSD/4, the alignment control stage and the sample/buffer control stage. Thus the experiment efficiency is improved. Results: The scattering curves and gyration radius of lysozyme under stationary collection mode and peristaltic collection mode were measured by experiments carried out at biological small angle X-ray scattering beamline of Shanghai Synchrotron Radiation Facility (SSRF). And results show that this device can prevent radiation damage of sample efficiently. Conclusion:The designed functionalities have been achieved as expected.%利用同步辐射X射线小角散射技术可以研究溶液中蛋白质的结构信息。但是在实验过程中，高通量的X 射线易造成蛋白质的辐射损伤，发生结构变化。本文介绍了一种自动换样溶液样品蠕动装置，在实验过程中利用Hamilton的PSD/4注射泵控制样品上下运动，减小单位体积照射时间以降低X射线对蛋白质的辐射损伤。此外，通过对注射泵、准直调节台和样品/缓冲液支撑台的协调控制实现了自动换样、回样和清洗功能，提高了实验效率。在上海光源生物X射线小角散射实验站进行了实验，通过对静止模式和蠕动模式下溶菌酶的散射曲线及回旋半径的测量，表明该装置可达到很好的防辐射损伤效果，实现了预期的样品蠕动装置功能。
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