收集不同发育时期的斑马鱼胚胎,制备DIG标记的TS反义RNA探针,采用整体原位杂交方法研究胸苷酸合成酶(TS)基因在斑马鱼胚胎发育各期的时空表达状况.结果表明,在所取样的各个阶段,TS基因均有转录,但其部位不同,中囊胚过渡前后mRNA显现的部位仅存在于受精卵的动物极,10hpf时存在于整个胚胎的外围,至24hpf后集中到头部及其躯干部.112hpf在心脏部位强表达,其余部位表达相对较弱.%To analyze the expression pattern of thymidylate synthase (TS) of zebrafish Danio rerio embryogenesis, we collected zebrafish embryos at different phases and prepared TS digoxin-labeled RNA probe using whole mount in situ hybridization. We found that TS expression appeared at early zygote period, continued throughout the entire embryogenesis periods. Expression of TS was also discovered in different parts of the embryo: in the animal pole of the middle gastrula, the margin of whole embryo after 10 hours, in the head and somite after 24 hours post fertilization. After 112 hours post-fertilization, strong TS expression only in the heart. Therefore, TS plays important role in the regulation of zebrafish embryonic development.
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