In this paper, the finite volume method was adopted to establish cross cell separation model, and a cell screening simulation method was proposed based on light pressure difference for analysis of influence factors of cell screening in microfluidics.Based on laminar flow, fluid flow, particle tracking and wave optics theories, a cross type optical particle separation model was established by finite element analysis.Using light pressure difference technology, various factors affecting the separation of cells were studied, including particle diameter, laser power, temperature and fiber diameter.Furthermore, deflection distances of the particles in the fluid due to the optical radiation pressure were determined experimentally.The results show that in microfluidics, laser power, cell diameter and temperature (20 ℃) generally are proportional to deflection distance separately.When fiber diameter and cell diameter are in the same size, optical radiation pressure reaches the maximum.When laser acts on particles with diameter of 3 μm, 8 μm and 20 μm through fibers with diameters of 7 μm or 8 μm, radiation pressure reaches its maximum, thus choosing single mode fiber with diameter of 8 μm as an important optical device in the experiments.The above conclusion provides an reference for further study of precision of numerical simulation on cell screening factors.%本文采用有限体积法建立了交叉型细胞分离模型,提出了一种基于光压差分的细胞筛选仿真方法,分析微流体中细胞筛选的影响因素.基于层流、流体流动粒子追踪、波动光学理论,利用有限元分析法建立了一种交叉型光学颗粒分离模型,研究了利用光压差分技术分离细胞的各种影响因素,其中包括微粒直径,激光功率、温度、光纤直径,分析了微粒在流体中因光辐射压力作用下的偏移距离.实验结果表明:在微流体中,激光功率、细胞直径、温度(20 ℃)和偏移距离大体上成正比关系,光纤直径和细胞直径在大小相当的情况下光辐射压力能够达到最大值,当激光通过光纤作用于直径分别为3,8和20 μm的微粒时,光纤直径为7 μm或8 μm时光辐射压力最大,所以选用直径为8 μm的单模光纤作为一个重要的实验光学器件.所得结论为深入研究细胞筛选影响因素的数值仿真精度提供了参考与借鉴.
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