High Aluminum Tolerance of Rhodotorula sp.RS1 is Associated with Thickening of the Cell Wall Rather than Chelation of Aluminum Ions*1

WANG Chao; ZHAO Xue-Qiang; T.AIZAWA; M.SUNAIRI; SHEN Ren-Fang

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High Aluminum Tolerance of Rhodotorula sp.RS1 is Associated with Thickening of the Cell Wall Rather than Chelation of Aluminum Ions*1

机译：Rhodotorula sp.RS1的高铝耐受性与细胞壁增厚而不是铝离子的螯合有关* 1

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Aluminum (Al) is very toxic to many living organisms,including plants,animals and microorganisms.However,despite many studies on Al tolerance in plants,little has been reported concerning these mechanisms in microorganisms.In this study,a red yeast,which could tolerate Al3+ concentrations as high as 200 mmol L-1,was isolated from acidic soils,identified as Rhodotorula sp.and designated as RS1.As the medium compositions can greatly affect the responses of microorganisms to Al,two culture mediums,glucose medium (GM) and lysogeny broth medium containing soil extract (S-LBM),were used.During growth of RS1,the pH of medium decreased in GM but increased in S-LBM.These changes in the pH of the media were not induced by Al addition.No or little secretion of organic acids was observed in RS1 growth media.Importantly,the thickness of the cell walls and the ratio of cell wall to biomass of RS1 significantly increased in GM with high Al3+ concentrations.In the presence of 100 mmol Al L-1,78.0％ of the total Al of whole cells was present in the thickened cell walls.The Al in cell walls was mostly bound to OH,amide and CO groups of polysaccharides.These results suggest that thickening of the cell wall in response to the high Al3+ concentrations may play an important role in the high tolerance of RS1 to Al and that pH increase of the medium and chelation of Al ions are not involved in Al tolerance of this organism.

机译：铝对包括植物，动物和微生物在内的许多生物均具有剧毒作用。然而，尽管对植物的铝耐受性进行了许多研究，但对微生物中这些机制的报道却很少。在这项研究中，一种红色酵母可以从酸性土壤中分离出的耐Al3 +浓度高达200 mmol L-1，鉴定为Rhodotorula sp。，命名为RS1。由于培养基组成会极大地影响微生物对Al的反应，两种培养基，葡萄糖培养基（GM））和含有土壤提取物（S-LBM）的溶菌性肉汤培养基。在RS1生长期间，培养基的pH值在GM中降低，但在S-LBM中升高。这些培养基pH值的变化不是由添加Al引起的在高浓度Al3 +的GM中，RS1生长培养基中没有或几乎没有有机酸的分泌。重要的是，GM中RS1的细胞壁厚度和细胞壁与生物量的比率显着增加。 -1,78.0％的整个细胞的总Al存在于增厚的细胞壁中。细胞壁中的Al主要与多糖的OH，酰胺和CO基团结合，这些结果表明高Al3 +浓度下细胞壁的增厚可能发挥了作用。 RS1对铝的高耐受性起着重要作用，并且介质的pH升高和铝离子的螯合不涉及该生物体对铝的耐受性。

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《土壤圈（英文版）》 |2013年第1期|29-38|共10页
作者
WANG Chao; ZHAO Xue-Qiang; T.AIZAWA; M.SUNAIRI; SHEN Ren-Fang;
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State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008,China;

University of Chinese Academy of Sciences, Beijing 100049,China;

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008,China;

College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880,Japan;

College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880,Japan;

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008,China;

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High Aluminum Tolerance of Rhodotorula sp.RS1 is Associated with Thickening of the Cell Wall Rather than Chelation of Aluminum Ions*1

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