Temporal and Tissue-Specific Expression of Tomato 14-3-3 Gene Family in Response to Phosphorus Deficiency

XU Wei-Feng; SHI Wei-Ming; YAN Feng

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Temporal and Tissue-Specific Expression of Tomato 14-3-3 Gene Family in Response to Phosphorus Deficiency

机译：番茄14-3-3基因家族对磷缺乏的时空和组织特异性表达

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Plants adapt to phosphorus (P) deficiency through a complex of biological processes and many genes are involved.Tomato (Solanum lycopersicum L.'Hezuo906’) plants were selected to grown hydroponically to study the temporal and spatial gene expression patterns of the 14-3-3 gene family and their roles in response to P deficiency in tomato plants.Using real-time reverse-transcriptase polymerase chain reaction (RT-PCR),we investigated the expression profiles in different tissues (root,stem and leaf) at short-term and long-term P-deficient stress phases.Results revealed that i) four members of 14-3-3 gene family (TFT1,TFT4,TFT6 and TFT7)were involved in the adaptation of tomato plants to P deficiency,ii) TFT7 responded quickly to P deficiency in the root,while TFT6 responded slowly to P deficiency in the leaf,iii) expression response of TFT4 to P-deficient stress was widely distributed in different tissues (root,stem and leaf) while TFT8 only displayed stem-specific expression,and iv) temporal and tissues-specific expression patterns to P deficiency suggested that isoform specificity existed in tomato 14-3-3 gene family.We propose that TFT7 (one member of ε-like group in tomato 14-3-3 family) is the early responsive gene and may play a role in the adaptation of tomato plants to short-term P deficiency,while TFT6 (one member of non-ε group in tomato 14-3-3 family) is the later responsive gene and may play a role in the adaptation of tomato plants to long-term P deficiency.

机译：植物通过复杂的生物过程适应磷（P）缺乏症，涉及许多基因。选择番茄（Solanum lycopersicum L.'Hezuo906'）植物进行水培生长，研究14-3的时空基因表达模式-3基因家族及其在番茄植株对磷缺乏的响应中的作用。使用实时逆转录聚合酶链反应（RT-PCR），我们研究了短时间在不同组织（根，茎和叶）中的表达谱。结果表明：i）14-3-3基因家族的四个成员（TFT1，TFT4，TFT6和TFT7）参与了番茄植物对P缺乏的适应，ii）TFT7根对P缺乏反应迅速，而TFT6对叶中P缺乏反应缓慢，iii）TFT4对P缺乏胁迫的表达反应广泛分布在不同组织（根，茎和叶）中，而TFT8仅显示茎-具体表达方式，以及iv）节奏磷缺乏的组织和组织特异性表达模式表明番茄14-3-3基因家族中存在同工型特异性。我们建议TFT7（番茄14-3-3家族中ε样成员之一）是早期反应该基因可能在番茄植物适应短期磷缺乏中发挥作用，而TFT6（番茄14-3-3家族非ε组成员之一）是较晚的响应基因，可能在番茄中番茄植物对长期缺磷的适应性。

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《土壤圈（英文版）》 |2012年第6期|735-745|共11页
作者
XU Wei-Feng; SHI Wei-Ming; YAN Feng;
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作者单位

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008(China);

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008(China);

Institute of Crop Science and Plant Breeding, Justus-Liebig University, Ludwigstrasse 23, 35390 Giessen(Germany);

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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Temporal and Tissue-Specific Expression of Tomato 14-3-3 Gene Family in Response to Phosphorus Deficiency

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