Expression Patterns of Nine Ammonium Transporters in Rice in Response to N Status

LI Su-Mei; LI Bao-Zhen; SHI Wei-Ming

摘要

Nitrogen use efficiency (NUE) was very low in China and a loss of as much as 70％ of the applied nitrogen fertilizers was reported in high-yielding rice fields.In order to investigate the molecular basis of high-affinity ammonium transport or uptake into rice (Oryza sativa L.),we analyzed the expression profiles of nine ammonium transporters (AMT),three each of OsAMT1,OsAMT2 and OsAMT3,at two different N requirement stages (young seedling stage and tillering stage) of rice growth as well as the changes in these expression patterns according to external N status using real-time reverse transcription polymerase chain reaction (RT-PCR).The results suggested that the nine OsAMT genes were expressed in different organs of rice plants,including mature roots,new roots,stems,old leaves and new leaves and that the expression patterns were organ specific and independent of the positions of the corresponding proteins in the phylogenetic tree.OsAMT1;1,3;2 and 3;3 were expressed in the roots and shoots,primarily old leaves,OsAMT1;2 and 1;3 mainly in the roots,and OsAMT2;1,2;2,2;3 and 3;1 mainly in the shoots,primarily in new leaves,and relatively more in the stems than other genes.The expression patterns at the two different N requirement stages were the same; however,at the tillering stage with greater N requirements,the OsAMTs transcript levels were greater than those at the young seedling stage with low N requirements.N starvation for 48 h up-regulated OsAMT1;1,1;2,3;1,3;2,3;3 and down-regulated OsAMT1;3 mRNA abundance. Following N starvation,NH4+ and NH4NO3 re-supply down-regulated OsAMT1;2 and 3;3 and up-regulated OsAMT1;3,whereas NO3- re-supply down-regulated OsAMT1;1 and 1;2.These suggested that the organ-specific expression pattern of OsAMT could be regulated by N requirement and external N status.

机译：中国的氮素利用效率很低，据报道在高产稻田中氮肥损失高达70％。为了研究高亲和性铵盐运移或吸收的分子基础。水稻（Oryza sativa L.），我们分析了9个铵转运蛋白（AMT）的表达谱，分别分析了水稻生长中两个不同的氮需求阶段（幼苗期和分er期）中的三个OsAMT1，OsAMT2和OsAMT3的表达谱。实时逆转录聚合酶链反应（RT-PCR）根据外部氮的状态改变这些表达方式。结果表明9个OsAMT基因分别在水稻的不同器官中表达，包括成熟根，新根，茎，老叶和新叶，其表达模式是器官特异性的，并且与系统发育树中相应蛋白质的位置无关.OsAMT1; 1,3; 2和3; 3在根和茎中表达OTs，主要是老叶，OsAMT1; 2和1; 3主要在根部，而OsAMT2; 1,2; 2,2; 3和3; 1主要在枝条中，主要在新叶中，并且相对较多在茎中在两个不同的氮需求阶段，表达模式是相同的。但是，在分N期氮需求较高的情况下，OsAMTs的转录水平要高于在低氮需求下的幼苗期的水平。N饥饿48小时后OsAMT1; 1,1; 2,3; 1,3 ; 2,3; 3和OsAMT1; 3 mRNA的表达下调。饥饿后，NH4 +和NH4NO3再次下调OsAMT1; 2和3; 3并上调OsAMT1; 3，而NO3再次下调OsAMT1; 1和1; 2。这表明器官OsAMT的特异性表达模式可通过N需求和外部N状态来调节。

Expression Patterns of Nine Ammonium Transporters in Rice in Response to N Status

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