• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>土壤圈（英文版） >Bacterial Community Structure in a Mollisol Under Long-Term Natural Restoration, Cropping, and Bare Fallow History Estimated by PCR-DGGE
【24h】

Bacterial Community Structure in a Mollisol Under Long-Term Natural Restoration, Cropping, and Bare Fallow History Estimated by PCR-DGGE

机译：通过PCR-DGGE估算的长期自然恢复，播种和裸露休闲史下的软体动物细菌群落结构

获取原文
获取原文并翻译 | 示例
       
页面导航
  • 摘要
  • 著录项
  • 相关主题

摘要

Soil microbial biomass and community structures are commonly used as indicators for soil quality and fertility.A investigation was performed to study the effects of long-term natural restoration,cropping,and bare fallow managements on the soil microbial biomass and bacterial community structures in depths of 0-10,20-30,and 40-50 cm in a black soil (Mollisol).Microbial biomass was estimated from chloroform fumigation-extraction,and bacterial community structures were determined by analysis of 16S rDNA using polymerase chain reaction-denaturing gradient gel electrophoresis (PCRDGGE).Experimental results showed that microbial biomass significantly declined with soil depth in the managements of restoration and cropping,but not in the bare fallow.DGGE profiles indicated that the band number in top 0-10 cm soils was less than that in depth of 20-30 or 40-50 cm.These suggested that the microbial population was high but the bacterial community structure was simple in the topsoil.Cluster and principle component analysis based on DGGE banding patterns showed that the bacterial community structure was affected by soil depth more primarily than by managements,and the succession of bacterial community as increase of soil depth has a similar tendency in the three managements.Fourteen predominating DGGE bands were excised and sequenced,in which 6 bands were identified as the taxa of Verrucomicrobia,2 bands as Actinobacteria,2 bands as α-Proteobacteria,and the other 4 bands as δ-Proteobacteria,Acidobacteria,Nitrospira,and unclassified bacteria.In addition,the sequences of 11 DGGE bands were closely related to uncultured bacteria.Thus,the bacterial community structure in black soil was stable,and the predominating bacterial groups were uncultured.
机译：土壤微生物的生物量和群落结构通常被用作指示土壤质量和肥力的指标。进行了一项调查，研究长期自然恢复，耕作和裸休管理对深部土壤微生物生物量和细菌群落结构的影响。在黑土（Mollisol）中0-10、20-30和40-50 cm处。通过氯仿熏蒸提取法估算微生物生物量，并使用聚合酶链反应-变性梯度凝胶分析16S rDNA以确定细菌群落结构实验结果表明，在恢复和耕作管理中，微生物量随土壤深度的增加而显着下降，但在裸露的休耕地中却没有。DGGE谱表明，0-10 cm土层中的带数少于土壤中的带数。深度为20-30厘米或40-50厘米，这表明表层土壤中微生物种群较高，但细菌群落结构简单。基于DGGE谱带模式的初步成分分析表明，细菌群落结构受土壤深度的影响大于受管理的影响，并且随着土壤深度的增加，细菌群落的演替在三种管理中都有相似的趋势。十四个主要的DGGE带是切下并测序，其中6条带被鉴定为Verrucomicrobia的类群，2条带被认为是放线菌，2条带被认为是Proteobacteria，另外4条带被称为δ-Proteobacteria，Acidobacteria，Nitrospira和未分类细菌。 11个DGGE带的序列与未培养细菌密切相关。因此，黑土中细菌群落结构稳定，主要细菌群未培养。

著录项

  • 来源
    《土壤圈（英文版）》 |2009年第2期|156-165|共10页
  • 作者

    WANG Guang-Hua; JIN Jian; LIU Jun-Jie; CHEN Xue-Li; LIU Ju-Dong; LIU Xiao-Bing;

  • 作者单位

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

    Key Laboratory of Black Soil Ecology,Northeast Institute of Geography and Agreecology,Chinese Academy of Sciences,Harbin 150081 China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 农业基础科学;
  • 关键词

获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号