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首页> 外文期刊>土壤圈（英文版） >Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethyl
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Characterization of a strain capable of degrading a herbicide mixture of quinclorac and bensulfuronmethyl

机译：能够降解喹克拉克和苄嘧磺隆的除草剂混合物的菌株的表征

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A bacterial strain,designated as LS,was isolated from a contaminated soil and was found to be capable of utilizing quinelorac,bensulfuromnethyl,and a mixture of the two as carbon and energy sources for growth.Strain LS was identified as Ochrobactrum sp.based on its physiological-biochemical properties,16S rDNA sequences,and phylogenetic analysis.The extent of degradation of quinclorac and bensulfuronmethyl at initial concentrations of 1.5 and 0.1 g L-1 was 90% and 67%,respectively,as measured by high performance liquid chromatography (HPLC).When a herbicide mixture of 0.34 g L-1 quinelorac and 0.02 g L-1 bensulfuronmethyl was applied as carbon sources,quinclorac and bensulfuronmethyl were degraded at 95.7% and 67.5%,respectively.It appears that quinclorac is utilized more easily in a mixture than in a single state.The optimal temperature for growth of strain LS was 37℃.Strain LS grew well at pH 6 to 9 and had the highest degradation level for quinclorac and bensulfuronmethyl at an initial pH of 7 and 8,respectively.Addition of 0.25 g L-1 yeast extract could promote the growth and extent of degradation of quinclorac and bensulfuronmethyl by strain LS.Strain LS also showed the capability to degrade other aromatic compounds such as catechol,propisochlor,4-chloro-2-methylphenoxyacetic acid sodium (MCPA-Na) and imazethapy.The isolate LS shows a huge potential to be used in bioremediation for treating complex herbicide residues.
机译：从污染的土壤中分离出一种命名为LS的细菌菌株，发现该菌株能够利用喹诺拉克，苯磺酰氨基乙以及两者的混合物作为碳和能源进行生长。高效液相色谱法测定，初始浓度为1.5和0.1 g L-1时，喹克洛拉和苄嘧磺隆的降解程度分别为90％和67％（16S rDNA序列和系统发育分析）。当使用0.34 g L-1喹诺拉克和0.02 g L-1苯磺隆甲基的除草剂混合物作为碳源时，奎克洛拉克和苄嘧磺隆的降解率分别为95.7％和67.5％。菌株LS的最佳生长温度为37℃。菌株LS在pH为6〜9时生长良好，喹氯喹和苄嘧磺隆的降解率最高。分别在7和8的pH值下。添加0.25 g L-1酵母提取物可以促进喹索拉克和苄嘧磺隆的生长以及LS菌株降解的程度.LS LS还具有降解其他芳香族化合物如邻苯二酚，异丙氯的能力。 ，4-氯-2-甲基苯氧基乙酸钠（MCPA-Na）和咪唑替比。分离物LS具有巨大的潜力，可以用于生物修复复杂的除草剂残留物。

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