Effect of nitrate on activities and transcript levels of nitrate reductase and glutamine synthetase in rice

CAO Yun; FAN Xiao-Rong; SUN Shu-Bin; XU Guo-Hua; HU Jiang; SHEN Qi-Rong

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Effect of nitrate on activities and transcript levels of nitrate reductase and glutamine synthetase in rice

机译：硝酸盐对水稻硝酸盐还原酶和谷氨酰胺合成酶活性及转录水平的影响

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Real-time polymerase chain reaction analysis was used to compare the effect of NO-3 on the activities of nitrate reductase (NR) and glutamine synthetase (GS),and the transcript levels of two NR genes,OsNia1 and OsNia2,two cytceolic GS1 genes,OsGln1;1 and OsGln1;2,and one plastid GS2 gene OsGln2,in two rice (Oryza sativa L.) cultivars Nanguang (NG) and Yunjing (YJ).Both cultivars achieved greater biomass and higher total N concentration when grown in a mixed N supply than in sole NH+ nutrition.Supply of NO-3 increased NR activity in both leaves and roots.Expression of both NR genes was also substantially enhanced and transcript levels of OsNia2 were significantly higher than those of OsNia1.NO-3 also caused an increase in GS activity,but had a complex effect on the expression of the three GS genes.In roots,the OsGln1;1 transcript increased,but OsGln1;2 decreased.In leaves,NO-3 had no effect on the GS1 expression,but the transcript for OsGln2 increased both in the leaves and roots of rice with a mixed supply of N.These results suggested that the increase in GS activity might be a result of the complicated regulation of the various GS genes.In addition,the NO-3 induced increase of biomass,NR activity,GS activity,and the transcript levels of NR and GS genes were proportionally higher in NG than in YJ,indicating a stronger response of NG to NO-3 nutrition than YJ.

机译：实时聚合酶链反应分析用于比较NO-3对硝酸还原酶（NR）和谷氨酰胺合成酶（GS）的活性以及两个NR基因OsNia1和OsNia2和两个细胞周期GS1基因的转录水平的影响水稻（Oryza sativa L.）的两个品种南光（NG）和云景（YJ）的OsGln1、1和OsGln1; 2以及一个质体GS2基因OsGln2。氮的供应量要比单独的NH +营养高.NO-3的供应增加了叶片和根部的NR活性。两个NR基因的表达也显着增强，OsNia2的转录水平显着高于OsNia1.NO-3也引起GS活性增加，但对这三个GS基因的表达具有复杂的影响。在根中，OsGln1; 1转录本增加，而OsGln1; 2减少。在叶片中，NO-3对GS1表达无影响，但是OsGln2的转录本在ric的叶和根中都增加了这些结果表明GS活性的增加可能是各种GS基因调控复杂的结果。此外，NO-3诱导生物量，NR活性，GS活性和NG中的NR和GS基因的转录水平成比例地高于YJ，表明NG对NO-3营养的响应要强于YJ。

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来源
《土壤圈（英文版）》 |2008年第5期|664-673|共10页
作者
CAO Yun; FAN Xiao-Rong; SUN Shu-Bin; XU Guo-Hua; HU Jiang; SHEN Qi-Rong;
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作者单位

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095 China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类农业基础科学;
关键词
ammonium; glutamine synthetase; nitrate; nitrate reductase; Oryza sativa L;

机译：铵;谷氨酰胺合成酶;硝酸盐;硝酸还原酶;稻;

Effect of nitrate on activities and transcript levels of nitrate reductase and glutamine synthetase in rice

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