Characterization of Bacterial Community Structure and Diversity in Rhizosphere Soils of Three Plants in Rapidly Changing Salt Marshes Using 16S rDNA

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Characterization of Bacterial Community Structure and Diversity in Rhizosphere Soils of Three Plants in Rapidly Changing Salt Marshes Using 16S rDNA

机译：快速变化盐沼中三种植物根际土壤细菌群落结构和多样性的16S rDNA表征

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The structure and diversity of the bacterial communities in rhizosphere soils of native Phragmites australis and Scirpus mariqueter and alien Spartina alterniflora in the Yangtze River Estuary were investigated by constructing 16S ribosomal DNA (rDNA) clone libraries. The bacterial diversity was quantified by placing the clones into operational taxonomic unit (OTU) groups at the level of sequence similarity of ＞ 97%. Phylogenetic analysis of the resulting 398 clone sequences indicated a high diversity of bacteria in the rhizosphere soils of these plants. The members of Alphaproteobacteria,Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria of the phylum Proteobacteria were the most abundant in rhizobacteria. Chao 1 nonparametric diversity estimator coupled with the reciprocal of Simpson's index (1/D) was applied to sequence data obtained from each library to evaluate total sequence diversity and quantitatively compare the level of dominance. The results showed that Phragmites, Scirpus, and Spartina rhizosphere soils contained 200, 668, and 382 OTUs, respectively. The bacterial communities in the Spartina and Phragmites rhizosphere soils displayed species dominance revealed by 1/D, whereas the bacterial community in Scirpus rhizosphere soil had uniform distributions of species abundance. Overall, analysis of 16S rDNA clone libraries from the rhizosphere soils indicates that the changes in bacterial composition may occur concomitantly with the shift of species composition in plant communities.

机译：通过构建16S核糖体DNA（rDNA）克隆文库，研究了长江口天然芦苇，Scirpus mariqueter和互生互花米草的根际土壤细菌群落的结构和多样性。通过将克隆按> 97％的序列相似性水平放置到操作分类单元（OTU）组中来定量细菌多样性。对所得398个克隆序列的系统进化分析表明，这些植物的根际土壤中细菌的多样性很高。杆状杆菌属的细菌中，杆状细菌，β-变形杆菌，γ-变形杆菌和δ-变形杆菌的成员最丰富。将Chao 1非参数多样性估计量与Simpson指数（1 / D）的倒数相结合，用于从每个库中获得的序列数据，以评估总序列多样性并定量比较优势水平。结果表明芦苇，Scirpus和Spartina根际土壤分别含有200、668和382 OTU。 Spartina和Phragmites根际土壤中的细菌群落显示出1 / D揭示的物种优势，而Scirpus根际土壤中的细菌群落具有均匀的物种丰度分布。总体而言，对根际土壤中16S rDNA克隆文库的分析表明，细菌组成的变化可能与植物群落中物种组成的变化同时发生。

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《土壤圈（英文版）》 |2007年第5期|545-556|共12页
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Coastal Ecosystems Research Station of Yangtze River Estuary, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, 220 Handan Road, Shanghai 200433,China;

Coastal Ecosystems Research Station of Yangtze River Estuary, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, 220 Handan Road, Shanghai 200433,China;

Coastal Ecosystems Research Station of Yangtze River Estuary, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, Fudan University, 220 Handan Road, Shanghai 200433,China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
原文格式 PDF
正文语种 chi
中图分类农业基础科学;
关键词
16S rDNA, bacterial diversity, plant succession, rhizosphere, salt marshes;

机译：16S rDNA;细菌多样性;植物演替;根际;盐沼;
入库时间 2022-08-19 04:04:16

Characterization of Bacterial Community Structure and Diversity in Rhizosphere Soils of Three Plants in Rapidly Changing Salt Marshes Using 16S rDNA

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