Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.

ZHANG Xiao-Hua; ZHANG Guo-Shun; ZHANG Zhong-Hui; XU Jian-Hong; LI Shun-Peng

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Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.

机译：ch骨双歧杆菌降解敌敌畏的菌株DDV-1的分离与鉴定。

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The objective of this research was to isolate a dichlorvos (2,2-dichlorovinyl dimethyl phosphate)-degrading strain of Ochrobactrum sp., and determine its effectiveness in remediation of a dichlorvos-contaminated soil. A dichlorvos-degrading bacterium (strain DDV-1) was successfully isolated and identified as an Ochrobactrum sp. based on its 16S rDNA sequence analysis. Strain DDV-1 was able to utilize dichlorvos as a sole carbon source, and the optimal pH and temperature for its cell growth and degradation were 7.0 and 30 ℃, respectively. Also, the growth and degradation of strain DDV-1 showed the same response to dissolved oxygen. In addition, the soil degradation test indicated that in soil spiked with 100 mg L-1 or 500 mg L-1 dichlorvos and inoculated with 0.5% or 1.0% (v/v) strain DDV-1, complete degradation of dichlorvos could be achieved in 24 h. The present study showed that strain DDV-1 was a fast dichlorvos-degrading bacterium in soil. However, further research will be needed to clarify the degradation pathway and the properties of the key enzymes involved in its biodegradation.

机译：这项研究的目的是分离Ochrobactrum sp。的一株降解敌敌畏（2,2-二氯乙烯基二甲基磷酸酯）的菌株，并确定其对被敌敌畏污染的土壤进行修复的有效性。成功地分离了敌敌畏降解细菌（菌株DDV-1），并将其鉴定为Ochrobactrum sp。基于其16S rDNA序列分析。 DDV-1菌株能够利用敌敌畏作为唯一碳源，其细胞生长和降解的最佳pH和温度分别为7.0和30℃。同样，菌株DDV-1的生长和降解显示出对溶解氧的相同反应。此外，土壤降解测试表明，在掺有100 mg L-1或500 mg L-1敌敌畏的土壤中接种0.5％或1.0％（v / v）菌株DDV-1，可以实现敌敌畏的完全降解。在24小时内本研究表明，DDV-1菌株是土壤中快速降解敌敌畏的细菌。但是，将需要进一步的研究来阐明其生物降解过程中的降解途径和关键酶的性质。

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来源
《土壤圈（英文版）》 |2006年第1期|64-71|共8页
作者
ZHANG Xiao-Hua; ZHANG Guo-Shun; ZHANG Zhong-Hui; XU Jian-Hong; LI Shun-Peng;
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作者单位

Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjin9 Agricultural University, Nanjing 210095 (China);

Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400 (China);

Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjin9 Agricultural University, Nanjing 210095 (China);

Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjin9 Agricultural University, Nanjing 210095 (China);

Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjin9 Agricultural University, Nanjing 210095 (China);

Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjin9 Agricultural University, Nanjing 210095 (China);

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收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 chi
中图分类农业基础科学;
关键词
degradation, dichlorvos, 16S rDNA, soiltest, strain DDV-1;

机译：降解;敌敌畏;16S rDNA;土壤测试;菌株DDV-1;

Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.

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