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Tomato Growth and Organic Acid Changes in Response to Partial Replacement of NO-3-N by NH+4-N

机译:NH + 4-N替代NO-3-N对番茄生长和有机酸变化的影响

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A hydroponic experiment was conducted to study the effect of partial replacement of NO-3-N by NH4+-N on the seedling growth and organic acid content of tomato (Lycopersicon esculentum Mill.). A completely randomized design was established with three replications and five treatments, i.e., NO-3-N/NH4+-N of 100/0, 75/25, 50/50, 25/75 and 0/100. Results showed that 25% replacement of NO3--N by NH4+-N significantly (P = 0.05) improved fresh and dry weight, revealing that a proper percentage of NH4+-N was important for tomato nitrogen nutrition. This could increase the plant growth even though tomato was a crop that preferred nitrate nutrition. Also an increase in the proportion of NH4+-N in the nutrient solution led to a significant decrease (P = 0.05) in malate, citrate and fumarate. However, the 25% NH4+-N plus 75% NO3--N treatment had no significant effect (P = 0.05) on the 2-ketoglutarate, succinate or oxalic acid content, showing that only some organic acids in tomato plants were affected. Only pyruvate increased significantly (P = 0.05), and it only increased for 25% and 50% replacement of NO3--N by NH4+-N. Metabolism of these organic acids, especially malate, citrate and fumarate, should be further studied at the molecular level in vegetables applied with different nitrogen forms.
机译:进行了水培试验,研究了用NH4 + -N替代NO-3-N对番茄幼苗生长和有机酸含量的影响。通过三个重复和五个处理(即NO-3-N / NH4 + -N为100 / 0、75 / 25、50 / 50、25 / 75和0/100)建立了完全随机的设计。结果表明,用NH4 + -N替代25%的NO3--N(P = 0.05)可改善鲜重和干重,这表明适当比例的NH4 + -N对番茄氮素营养很重要。即使番茄是喜欢硝酸盐营养的农作物,这也可以增加植物的生长。营养液中NH4 + -N比例的增加还导致苹果酸,柠檬酸和富马酸的显着降低(P = 0.05)。然而,25%NH4 + -N加75%NO3--N处理对2-酮戊二酸,琥珀酸或草酸含量无显着影响(P = 0.05),表明番茄植株中只有一些有机酸受到影响。仅丙酮酸显着增加(P = 0.05),并且仅在NH4 + -N替代NO3--N的25%和50%时才增加。这些有机酸的代谢,尤其是苹果酸,柠檬酸和富马酸,应在分子水平上对不同氮素形式的蔬菜进行代谢研究。

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