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QTLs for Rice Leaf Chlorophyll Content Under Low N Stress

机译:低氮胁迫下水稻叶片叶绿素含量的QTL

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Quantitative trait loci (QTLs) for chlorophyll content of a rice leaf were mapped on to the molecular marker linkage nutrient solution and soil culture experiments to detect rice nitrogen nutrition status under low N stress. A chlorophyll meter was used to measure the soil plant analysis development (SPAD) value of the topmost fully expanded leaf as the index of chlorophyll content that expressed nitrogen status in rice plants. Totally 3 QTLs for SPAD values, two on chromosome 3 located at interval RG179-CDO337 and RG348-RZ329, respectively, and one on chromosome 10 at interval RZ500-RG134, were detected under stressed conditions of low N in the soil and/or nutrient solution culture experiments.One QTL located at interval RG179-CDO337 on chromosome 3 associated with a relative change in SPAD value from a high N level to a low N level in the soil culture experiment was also detected. Based on the different responses to low N stress between the two parents, it was supposed that the QTLs identified in this study associated with nitrogen efficiency in rice at low N levels might be useful in applying marker technology to rice breeding programs.
机译:将水稻叶片叶绿素含量的数量性状基因座(QTL)定位到分子标记连锁营养液上,并进行土壤培养实验,以检测低氮胁迫下水稻的氮素营养状况。叶绿素仪用于测量最顶部完全展开的叶的土壤植物分析发育(SPAD)值,作为表示水稻植物氮素状态的叶绿素含量指标。在土壤和/或养分含量低的胁迫条件下,共检测到3个SPAD值QTL,两个位于3号染色体RG179-CDO337和RG348-RZ329上,一个位于10号染色体RZ500-RG134间隔上。在土壤培养实验中,还检测到一个位于3号染色体RG179-CDO337区间的QTL,该SPTL值与SPAD值从高N水平到低N水平的相对变化有关。基于两个亲本对低氮胁迫的不同反应,可以认为本研究中确定的与低氮水平水稻氮效率相关的QTL可能对将标记技术应用于水稻育种计划有用。

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