Some Limitations of BIOLOG System for Determining Soil Microbial Community

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Some Limitations of BIOLOG System for Determining Soil Microbial Community

机译：BIOLOG系统确定土壤微生物群落的某些局限性

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A laboratory experiment was conducted to evaluate the effect of triphenyltetrazolium chloride (TTC)on soil microorganisms and the availability of pH characterization medium in BIOLOG plates. Applicationof TTC decreased the color development sharply and resulted in a great biocidal effect on the growth andreproduction of soil microorganisms, indicating that TTC can affect the discrimination on soil microbialcommunity. The microtitration plates with 21 carbon sources and two different pH levels (4.7 and 7.0) wereused to determine microbial community structure of eight red soils. The average utilization (average wellcolour development) of the carbon sources in the plates with different pH levels generally followed the samesigmoidal pattern as that in the traditional BIOLOG plates, but the pH 4.7 plates increased the discrimination of this technique, compared with the pH 7.0 plates. Since most tested soils are acid, it seemed that it's better to use a suitable pH characterization medium for a specific soil in the sole carbon source test.

机译：进行了一项实验室实验，以评估三苯基氯化四氮（TTC）对土壤微生物的影响以及BIOLOG板中pH表征培养基的可用性。 TTC的应用显着降低了颜色的发展，对土壤微生物的生长和繁殖产生了很大的杀菌作用，表明TTC可以影响对土壤微生物群落的区分。用具有21个碳源和两种不同pH值（4.7和7.0）的微量滴定板测定8种红壤的微生物群落结构。 pH值不同的印版中碳源的平均利用率（平均显色性）通常遵循与传统BIOLOG印版相同的S型曲线，但pH 4.7的印版与pH 7.0的印版相比，增加了该技术的辨别力。由于大多数被测试的土壤都是酸性的，因此似乎最好在唯一的碳源测试中针对特定的土壤使用合适的pH表征介质。

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《土壤圈（英文版）》 |2000年第1期|37-44|共8页
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Department of Soil Science and Agricultural Chemistry, Zhejiang University, Hangzhou 310029 (China);

Department of Soil Science and Agricultural Chemistry, Zhejiang University, Hangzhou 310029 (China);

Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH (UK);

Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH (UK);

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收录信息中国科学引文数据库(CSCD);
原文格式 PDF
正文语种 chi
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关键词
BIOLOG; microbial community; red soil; triphenyltetrazolium chloride;

机译：生物日志;微生物群落;红壤;氯化三苯四唑;

Some Limitations of BIOLOG System for Determining Soil Microbial Community

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