Ectomycorrhizal and Saprotrophic Fungal Communities Vary Across mm-Scale Soil Microsites Differing in Phosphatase Activity

Aaron GODIN; Denise BROOKS; Sue J.GRAYSTON; Melanie D.JONES

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Ectomycorrhizal and Saprotrophic Fungal Communities Vary Across mm-Scale Soil Microsites Differing in Phosphatase Activity

机译：磷酸酶活性不同的毫米级土壤微站点上的菌根和腐生真菌群落各不相同

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To understand nutrient cycling in soils,soil processes and microorganisms need be better characterized.To determine whether specific trophic groups of fungi are associated with soil enzyme activity,we used soil imprinting to guide mm-scale sampling from microsites with high and low phosphatase activities in birch/Douglas-fir stands.Study 1 involved sampling one root window per site at 12 sites of different ages (stands);study 2 was conducted at one of the stem-exclusion stands,at which 5 root windows had been installed.Total fungal and ectomycorrhizal (EM) fungal terminal-restriction fragment length polymorphism (TRFLP)fingerprints differed between high-and low-phosphatase activity microsites at 8 of 12 root windows across 12 sites.Where differences were detected,fewer EM fungi were detected in high-than low-phosphatase activity microsites.Using 5 root windows at one site,next-generation sequencing detected similar fungal communities across microsites,but the ratio of saprotrophic to EM fungal reads was higher in high-phosphatase activity microsites in the two windows that had low EM fungal richness.In windows with differences in fungal communities,both studies indicated that EM fungi were less successful than saprotrophic fungi in colonizing fine-scale,organic matter-rich microsites.Fine-scale sampling linked with in situ detection of enzyme activity revealed relationships between soil fungal communities and phosphatase activity that could not be observed at the scales employed by conventional approaches,thereby contributing to the understanding of fine-scale phosphorus cycling in forest soils.

机译：为了更好地了解土壤中的养分循环，需要更好地表征土壤过程和微生物。为确定特定营养类真菌是否与土壤酶活性相关，我们使用土壤印迹技术从高和低磷酸酶活性的微地点进行毫米级采样桦木/道格拉斯冷杉林分。研究1涉及在12个不同年龄（林分）的站点上每个站点取样一个根窗;研究2在其中一个已安装5个根窗的茎排斥林中进行。高和低磷酸酶活性微位点在12个位点的12个根窗中有8个在高，低磷酸酶活性微位点和外生菌根（EM）真菌的末端指纹多态性指纹图谱上有所不同。低磷酸酶活性的微位点。在一个位点使用5个根窗口，下一代测序可在各个微位点检测到相似的真菌群落，但腐生与E的比率在具有低EM真菌丰富度的两个窗口中，高磷酸酶活性的微位点的M真菌读数较高。在真菌群落不同的窗口中，两项研究均表明EM真菌在定殖细小，有机物方面不如腐生真菌更成功精细采样与酶活性的原位检测相结合揭示了土壤真菌群落与磷酸酶活性之间的关系，这在常规方法所采用的规模下是无法观察到的，从而有助于了解土壤中细尺度的磷循环森林土壤。

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《土壤圈（英文版）》 |2019年第3期|344-359|共16页
作者
Aaron GODIN; Denise BROOKS; Sue J.GRAYSTON; Melanie D.JONES;
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作者单位

Biology Department, University of British Columbia, Okanagan campus, Kelowna V1V 1V7 Canada;

Department of Forest & Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver V6T 1Z4 Canada;

Department of Forest & Conservation Sciences, Faculty of Forestry, University of British Columbia, Vancouver V6T 1Z4 Canada;

Biology Department, University of British Columbia, Okanagan campus, Kelowna V1V 1V7 Canada;

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Ectomycorrhizal and Saprotrophic Fungal Communities Vary Across mm-Scale Soil Microsites Differing in Phosphatase Activity

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