An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

LI Xiao-Fang; YIN Hong-Bin; SU Jian-Qiang

首页> 中文期刊> 《土壤圈：英文版》 >An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Understanding the mechanisms of Cu pollution-induced community tolerance (PICT) in soil requires the characterization of Cu-resistant microorganisms at a community level using modern molecular tools. A primer pair (copAF2010 (5 -TGCAC CTGAC VGGSC AYAT-3 )/copAR2333 (5 -GVACT TCRCG GAACA TRCC-3 )) tentatively targeting Pseudomonas-like Cu-resistant microorganisms was designed in this study. The specification of the primers was tested through conventional polymerase chain reaction (PCR) and the construction of a Pseudomonas-like copA gene fragment library, and then the primers were used to quantify the Cu-resistant microorganisms using quantitative PCR technique. A significant increase of Cu-resistant microorganisms targeted by the primers was observed in a paddy soil from Jiaxing, China which has been exposed to one-year Cu contamination. The results provided direct evidence for Cu PICT in the soil, and the quantification method developed in this study has the potential to be used as a molecular assay for soil Cu pollution.

著录项

来源
《土壤圈：英文版》 |2012年第2期|201-205|共5页
作者
LI Xiao-Fang; YIN Hong-Bin; SU Jian-Qiang;
展开▼
作者单位

Key Lab of Urban Environment and Health;

Institute of Urban Environment;

Chinese Academy of Sciences;

Xiamen 361003(China);

Centre for Mined Land Rehabilitation;

Sustainable Minerals Institute;

the University of Queensland;

St Lucia Qld 4072(Australia);

Research Centre for Eco-Environmental Sciences;

Chinese Academy of Sciences;

Beijing 100085(China);

展开▼
原文格式 PDF
正文语种 chi
中图分类基因工程（遗传工程）;
关键词
抗微生物; 铜污染; 水稻土; 量化; 嘉兴; 中国; PCR技术; 聚合酶链反应;

An Attempt to Quantify Cu-Resistant Microorganisms in a Paddy Soil from Jiaxing, China

摘要

著录项

相关主题

期刊订阅