目的 两个化合物A和X采用液相色谱质谱联用法实现部分分离,并形成部分重叠峰.尝试在不改变分离条件的情况下获得化合物X的“较纯的”质谱图.方法 运用MassLynx 4.1软件的“谱图合并(Combine Spectra process)”功能,扣除化合物A的信号.结果 与预期相符,所获得的质谱图明晰,提示X可能是A的脱氢类似物,后经验证,与A脱氢类似物纯品的质谱图一致.结论Masslynx 4.1软件的“谱图合并”功能可以帮助分析者更容易地获得清晰的质谱信息,并有可能节省传统方法中优化色谱条件所花费的时间,是液相色谱质谱联用分析的有力工具.%Objective To obtain a "pure" mass spectrum of compound X without further optimization of the separation method. When two compounds A and X are partially separated with the current liquid chromatogra-phy/tandem mass spectrometry method and produce overlapped peaks. Methods The "Combine Spectra process" function of MassLynx 4.1 software with the subtraction of compound A's signal was used. Results As expected, the extracted mass spectrum was unambiguous and indicated that compound X was possibly a dehydrogenated analog of compound A, which was supported by the similarity between the mass spectrum of X and the subsequently obtained mass spectrum of pure dehydrogenated analog of A. Conclusion The "Combine Spectra process" function of Mass-Lynx 4.1 software can make it easier for the analyser to obtain clear mass spectrometric information, and may save the time taken to optimize the chromatographic condition by traditional methods. It is a powerful tool for high performance liquid chromatography analysis.
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