RPR1(rice probenazole-responsive)is a rice gene,the expression of which is responsive to probenazole(PBZ),a synthetic compound that may act as a plant defense activator.It has been shown that RPR1 gene may be involved in disease resistance responses.In this study,a series of amplified fragments from the rice RPR1 promoter region,including 2 416 bp,1 574 bp,819.bp,568 bp and 208 bp fragments upstream to the ATG translation start site,were prepared and linked to the coding region of β-glucuronidase(GUS)gene.Analysis of GUS gene transient expression in rice calli demonstrated that the 568 bp fragment was sufficient for probenazole responsiveness.Analysis of GUS gene stable expression in Arabidopsis thaliana indicated that the 2 416 bp and 1 574 bp fragments drove GUS expression only in shoot apical meristem and petiole.Identification of these PBZ-responsive fragments provides a basis on which PBZ-inducible gene regulatory systems Can be constructed for experimental analysis of gene expression and for field application.%烯丙异噻唑是一种合成化合物,可诱导植物产生广谱抗病性.水稻中的RPR1(rice probenazoleresponsive)基因可能参与了烯丙异噻唑诱导抗病的过程.本研究用5'端删除的方法克隆了RPR1基因的5个不同长度的启动子片段(2 416 bp、1 574 bp、819bp、568 bp、208 bp),将其分别与GUS基因融合后转化拟南芥和水稻愈伤组织.结果表明,除208bp片段外,其余各片段在水稻愈伤组织中可受烯丙异噻唑诱导而驱动GUS基因的表达;1 574 bp和2 416 bp序列在异位表达系统的拟南芥中可驱动GUS的表达,说明单子叶和双子叶植物在烯丙异噻唑诱导获得抗病性的过程中可能存在某些共同机制;另外,1 574.bp和2 416 bp片段只驱动GUS在拟南芥芽顶端分生组织和叶柄中表达,说明这2个片段中可能含有调控组织特异性表达的顺式作用元件.这些烯丙异噻唑应答片段的鉴定为构建可用于基因表达分析,特别是田间可诱导基因表达系统提供了基础.
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