首页> 中文期刊> 《植物病虫害研究:英文版》 >Study on Biological Characteristics and Toxicity Determination of Colletotrichum orbiculare Causing Anthracnose of Watermelon in Hainan Province

Study on Biological Characteristics and Toxicity Determination of Colletotrichum orbiculare Causing Anthracnose of Watermelon in Hainan Province

         

摘要

[Objective] This paper was aimed to study the biological characteristics of Colletotrichum orbiculare(Berk.et Mont.) Arx, and accomplish the preliminary screening the test of fungicides. [Method]The pathogen was isolated from the infected leaves of watermelon collected from greenhouse in Hainan Province. The biological characteristics of C. orbiculare and indoor toxicity of fungicides were studied. [Results] The mycelium grew fastest on the medium with leaf extract of watermelon. The optimum temperature for mycelial growth and spore germination was 28 and 30 ℃, and the optimum pH was 7-9 and 6-8, respectively. The continuous darkness condition was most suitable for mycelial growth. The lethal temperature of spore was 55 ℃ water bath for 10 min in water bath. The suitable carbon sources were maltose, L-arabinose and glucose, and the suitable nitrogen sources were yeast extract, beef extract and peptone. In the indoor toxicity determination, 32.5% Benzoic azoxystrobin SC, 50% Dichlorotetrakis WP and 10% Difenoconazole WG showed better inhibition effects against the mycelial growth of C. orbiculare, with the EC50 values of 0.018 0, 0.060 2 and 0.471 5 μg/ml, respectively. [Conclusion]The research provided theoretical basis for the control of watermelon anthracnose in watermelon fields.

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