The COMT gene mainly participates in the biosynthesis of S lignin.The content of lignin will reduce when the activity of COMT gene is suppressed in the transgenic plants.In this study,the BSP and COMT were inserted into the vector pCAM2301 to construct a recombinant expression gene.The re-combinant gene was transformed into fiber flax by Agrobacterium tumefacien mediated callus transforma-tion.The transformed calluses were subjected to kanamycin screening and resistance callus was induced in induction and differentiation culture.The transformed seedlings were obtained and verified by PCR. The COMT gene was integrated into genome of the flax.We gained the positive genetic flax would lay a solid foundation for the research of flax fiber and variety breeding.%COMT基因主要参与S木质素的生物合成，转基因植物在COMT活性被抑制时，木质素含量减少，利用杨树皮储藏蛋白（ BSP ）启动子和该基因构建成反义植物表达载体pBSP－anti－COMT－2301，利用农杆菌介导法将其转入亚麻，经过卡那霉素筛选获得了抗性的愈伤组织，诱导分化后获得了亚麻苗。经特异引物的PCR扩增，证明目的基因已经整合到亚麻基因组中，本研究获得的阳性转基因亚麻植株为亚麻纤维研究及品种选育奠定了坚实基础。
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