In order to identify the pathogen causing anthracnose of Mahonia fortunei,small pieces of tissues were taken from the leaves which exhibited symptoms of anthracnose,and through tissue separation,single-spore puri-fication and Koch's postulate,the pathogenic isolate was confirmed.Based on morphological characteristics and multi-locus sequences,the isolate was identified as Colletotrichum fructicola.The isolate had a wide range of tem-perature adaptation;the temperature range for mycelium growth and sporulation was from 10℃ to 35℃ and the optimum temperature was 30℃.The light had no remarkable effect on the mycelial growth and sporulation.The pH range for mycelium growth and sporulation was from 3 to 11,and the optimum pH for mycelium growth and sporulation was 7 and 4,respectively.The optimal carbon sources for mycelial growth were dextrose,mannitol, maltobiose and lactose,and for sporulation,maltobiose.The optimal nitrogen source for mycelial growth and sporulation was peptone.%为弄清狭叶十大功劳炭疽病的病原菌,从四川省成都市采集疑似为炭疽病的病叶,采用组织分离法分离病原菌,单孢纯化后通过柯赫氏法则验证为致病菌;根据形态学特征,结合多基因系统学,将病原菌鉴定为果生炭疽菌Col-letotrichum fructicola.该菌对温度的适应范围较广,10~35℃之间均能生长和产孢,最适生长和产孢温度均为30℃;光照对菌丝生长和产孢影响不显著;在pH 3~11之间均能生长和产孢,菌丝生长最适pH为7,产孢最适pH为4;葡萄糖、甘露醇、乳糖和麦芽糖是菌丝生长的最适碳源,麦芽糖为产孢的最适碳源;蛋白胨是生长和产孢的最适氮源.
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