Objective:AccordingtoAAX14401,whichistranslatedbyAY887902integrityreadingframe,preparationof antibody named anti-AY to AAX14401,and determinate the anti-AY. Methods:Amino acid translated by ORF of AY887902 showed significant deviations of GSTP1 at amino acid sequence 40 to 52 which we used as template,then according to the de-mand of preparation of antibodies,synthetic polypeptide and inject into mouse to make its polyclonal antibody:anti-AY. Re-sults:Enzyme-linked immunosorbent assay( ELISA)results reviewed that titer of the polyclonal is 1:35 000,which sug-gested that the polyclonal antibody was eligibility to detect AAX14401. Conclusion:The antibody of anti-AY prepared of pro-tein AAX14401 appropriate for the demand,and make a well foundation for detect the functional of AY887902.%目的：由AY887902完整读码框翻译的蛋白AAX14401，制备蛋白AAX14401相应抗体anti-AY，并对其进行鉴定。方法：以AY887902的ORF所翻译氨基酸与GSTP1相比差异较大的40~52位上氨基酸序列为模板，按照抗体制备要求，合成多肽并免疫小鼠，制备蛋白AAX14401多克隆抗体anti-AY。结果：经过用酶联免疫吸附剂测定法（ELISA）检测，抗体滴度为1：35000，滴度达到要求，可以进行蛋白 AAX14401的检测。结论：制备的蛋白AAX14401抗体anti-AY符合要求，为进一步对AY887902功能进行检测奠定了较好的基础。
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