Over the years, the specific ELISA for detection antibody to capsular polysaccharide ( PPS) of Streptococcus pneumoniae has experienced two major improvements .The paper introduces the WHO recommended quantitative ELISA ( Pn PS ELISA ) in determination of human serum IgG antibody to pneumococcal capsular polysaccharide with the key rea -gents, limitations in its application , and its verification ,etc.If the value of IgG antibody level in sera determinated by Pn PS ELISA is above protective threshold ,the sera can prevent of invasive disease in infants .The protective threshold pro-vides serological data in the registration and serves as the primary endpoint of the protective efficacy for pneumococcal vac -cine.%检测肺炎链球菌荚膜多糖（ PPS）特异性抗体的酶联免疫吸附试验多年来经历了两次主要改进。介绍了WHO推荐的人血清抗肺炎链球菌（Streptococcus pneumoniae）荚膜多糖抗体IgG定量ELISA（Pn PS ELISA）检测方法、关键试剂、局限性以及方法验证等内容。 ELISA定量检测法，为预防肺炎链球菌的婴幼儿侵袭性疾病提供准确的抗体水平，并对肺炎链球菌疫苗的临床评价提供血清学证据。人血清中肺炎链球菌荚膜多糖抗体IgG是判断肺炎链球菌疫苗效力的重要指标。
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