Objective: To investigate the role of SOCS-1 (Suppressor of Cytokine Signalling-1) in tumorigenesis of adult acute myeloid leukemia (AMDby detecting the methylation state and expression of SOCS-1 gene. Methods: A total of 24 patients with AML, 10 healthy volunteers and 4 cell lines (Jurkat, Raji, U 937, NALM 17) were chosen. The methylation state of SOCS-1 in samples and cell lines were detected by using methylation-specific polymerase chain reation (MSP), and the expression of SOCS-1 was detected by using Real-time PCR. Results: The aberrant methylation of SOCS-1 was 62.5 % (15/24 )in primary patients with AML. In contrast, there was no aberrant methylation of SOCS-1 gene in normal samples (P<0.05). The expression of SOCS-1 in AML patients with aberrant methylation of SOCS-1 gene was lower than that in AML patients without aberrant methylation of SOCS-1 gene (P<0.05). There was no relation between the methylation of SOCS-1 gene and the clinicopathological data (such as sex, age and tumor stage). Conclusion: The methylation of SOCS-1 gene in AML was higher than that in control group significantly (P<0.05), and aberrant methylation strongly correlates with reduced expression. SOCS-1 gene may play an important role in tumorigenesis of AML.%目的:通过检测成人急性髓性白血病中SOCS-1基因表达水平及其甲基化水平,研究其在白血病发病中的作用.方法:运用甲基化特异性PCR(Methylation specific PCR,MSP)方法,对24例急性髓性白血病患者和4株白血病细胞株(Jurkat、Raji、U 937、NALM 17),进行SOCS-1基因甲基化水平的研究；同时运用Real-time PCR法定量分析SOCS-1基因表达水平.以10例健康人为正常对照组.结果:24例成人急性髓性白血病患者中,15例有SOCS-1基因甲基化(62.5%),而正常对照组无SOCS-1基因甲基化(0%),二者有显著差异( P＜0.05)；SOCS-1基因甲基化组与无SOCS-1基因甲基化组相比较,其SOCS-1基因相对表达量明显减少(P口0.05)；与患者临床病理特征相结合比较,发现SOCS-1基因的甲基化与患者年龄、性别和病程阶段无相关.4株白血病细胞株中,Jurkat和U 937表现有SOCS-1甲基化(50%),Raji和NALM 17无SOCS-1甲基化,前者SOCS-1基因表达量较后者也明显降低(P＜0.05).结论:SOCS-1基因在成人急性髓性白血病中甲基化水平明显增高,且SOCS-1基因甲基化后表达水平受到抑制,提示SOCS-1基因及其甲基化在急性髓性白血病的发生发展中可能具有一定作用.
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