In order to understand the avian leukosis virus epidemic situation in Guangxi chickens,two strains of avian leukosis virus subgroup A and J were isolated in Three-yellow chickens in the cultured DF-1 cells,which were identified by ALV p2 7 detection and PCR amplification and sequence analysis of avian leukosis virus gp85 gene,and then were named as HG01-A and HG01-J,respectively.The results of varia-tion analysis of gp85 gene indicated that the homology between HG01-A and 7 reference strains was 85.8%-87.5%,and shared the highest homology with MQNCSU at 87.5%,shared the homology with RSA at 86.9%.The results of variation analysis of gp85 gene indicated that the homology between HG01-J and 7 reference strains was 84.0%-93.8%,and shared the highest homology withXX2-08 and SCSM01 at 92.8%,shared the homology withHPRS1-3 at 90.2%.The present results indicated that it the first report that there was the co-infection of ALV-A and ALV-J in the Guangxi Three-yellow chickens,and provided new epidemiological information of avian leucosis virus in local chicken.%为了解禽白血病病毒在广西鸡群中流行情况，采用 DF-1细胞接种、细胞培养上清 p27抗原检测、PCR扩增，对临床血管瘤型禽白血病的三黄鸡病料进行了病毒分离，并对分离病毒 gp85基因进行测序和序列比较。结果表明，从1只病鸡同时分离到了一株A亚群禽白血病病毒（ALV-A）与一株J亚群禽白血病病毒（ALV-J），分别命名为 HG01-A 株和 HG01-J 株。ALV-A gp85与7株 A 亚群氨基酸同源性为85．8％～87．5％，与 A 亚群美国株 MQNCSU 同源性最高为 87．5％，与 A 亚群原型株 RSA 同源性为86．9％。而ALV-J gp85与7株毒株核酸序列同源性为84．0％～93．8％，与广东株XX2-08以及四川株SC-SM01同源性最高为93．8％，与英国原型株 HPRS103同源性为90．2％。进化分析进一步表明，HG01-A与各参考株亲缘关系较远，H G01-J与SCSM01 亲缘关系最近。本研究首次从同一只广西三黄鸡中同时分离到ALV-A及ALV-J，进一步完善了我国地方品种鸡群中禽白血病的流行病学信息。
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