Objective An HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose, fructose, sucrose from Panax notoginseng. Methods The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water (75:25) at a flow rate of 0.8 mL/min by gradient elution. The ELSD drift tube temperature was set at 75 ℃ and the carrying gas was air. Results Under the above conditions, monosaccharides and disaccharides from Panax notoginseng could be well separated, and the spiked recoveries were 98.23%～101.46% with a RSD less than 3% (n = 3). Conclusion This method was accurate, rapid, simple and reproducible, and thus it would be suitable for the determination of monosaccharides, disaccharides from Panax notoginseng.%目的 建立高效波相色谱-蒸发光散射检测法,同时测定三七药材中单糖和二糖的含量.方法 HPLC采用Thermo Hypersil GOLD Amino氨基柱(250×4.6 mm),流动相为乙腈-水(75:25),流速0.8mL·min-1,ELSD漂移管温度75℃,载气为空气,载气流速2.5 L·min-1.结果 在该条件下三七药材中的单糖和二糖组分能够得到较好的分离,线性关系良好(相关系数R2＞0.99),回收率在98.23％～101.46％之间,且相对标准偏差小于3％.结论 该方法简便、准确、灵敏、稳定,适合于三七药材中单糖和二糖的含量测定.
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