Objective:To investigate the effects of Wenyang Huazhuo Tongluo Prescription(温阳化浊通络方)on the phenotype transformation and migration of human skin ifbroblast induced by Wnt-1.Methods:Medicated serum of Wistar rats was got by intragastric administration of Wenyang Huazhuo Tongluo Prescription and normal human skin fibroblasts were cultured in vitro.The rats were divided into a blank control group,a Wnt group and a Wenyang Huazhuo Tongluo Prescription group.Respectively after 24,48,72 hours,RT-PCR and Western blot were used to detect the expressions of α-SMA mRNA and protein,and Transwell was used to detect the migration of fibroblasts.Results:Compared with the blank control group,human skin fibroblasts a-SMA mRNA and protein expression in the Wnt group could be induced in different time(P<0.01),and the number of migration increased(P<0.01).Compared with the Wnt group,human skin ifbroblasts a-SMA mRNA and protein expression in the Wenyang Huazhuo Tongluo Prescription group were obviously inhibited(P < 0.05, P<0.01) and their migration were prevented(P<0.01).Conclusion:Medicated serum of Wenyang Huazhuo Tongluo Prescription can inhibit the phenotype transformation and migration of human skin ifbroblast induced by Wnt-1,having the potential to prevent or delay systemic sclerosis ifbrosis.%目的：探讨温阳化浊通络方含药血清对Wnt-1诱导的人皮肤成纤维细胞表型转化及迁移的影响。方法：通过对Wistar大鼠灌胃制备温阳化浊通络方含药血清，体外培养正常人皮肤成纤维细胞，分为空白对照组、Wnt组和温阳化浊通络方组，作用24，48，72 h后，分别用RT-PCR和Western blot法检测α-SMA mRNA及蛋白的表达，Transwell试验检测成纤维细胞迁移情况。结果：与空白对照组比较， Wnt组在不同作用时间下能诱导人皮肤成纤维细胞α-SMA mRNA及蛋白表达（P<0.01），增加其迁移数量（P<0.01）；与Wnt组比较，温阳化浊通络方组在不同作用时间下能够显著抑制Wnt-1诱导的人皮肤成纤维细胞α-SMA mRNA及蛋白表达（P<0.05，P<0.01），同时阻止其迁移（P<0.01）。结论：温阳化浊通络方含药血清能抑制Wnt-1诱导的人皮肤成纤维细胞表型转化及迁移，具有阻止或延缓系统性硬化病纤维化的潜能。
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