目的：通过观察健膝丸对膝骨关节炎模型SD大鼠血清SOD、MDA、NO表达水平的影响，以探求健膝丸在防治膝骨关节炎方面的作用机理，从而为本药的临床应用及药物开发提供科学的理论依据，同时进一步探索骨关节炎的发病机制。方法：以40只雌性SD大鼠为实验对象，随机分为正常组、模型对照组、健膝丸组、抗骨增生胶囊组，每组10只。除正常组外，其余各组均采用改良Hulth法对大鼠进行造模。术后连续3 d肌肉注射青霉素20万U，1周后开始强迫大鼠每天活动0.5 h，连续8周。同时各组大鼠均采用灌胃给药途径给予相应药液，根据人与动物体表面积等效剂量折算出健膝丸组及抗骨增生胶囊组灌胃给药量。正常组及模型对照组给予质量浓度0.9％的生理盐水，连续8周。8周后分别抽取各组大鼠腹腔静脉血，离心后取上清，按照SOD、MDA、NO测试盒说明操作，用754分光光度计测各管吸光度（OD值），从而进一步计算出各组血清中的各指标的含量。结果：血清中SOD、MDA、NO含量测定结果显示，模型对照组与正常组比较，模型对照组血清中SOD活性显著下降（P<0.01），MDA、NO含量显著升高（P<0.01）。健膝丸组与正常组比较，SOD活性差异无统计学意义（P>0.05），MDA及NO含量差异有统计学意义（P<0.05）。健膝丸组与模型对照组比较，SOD活性显著升高（P<0.01），MDA含量差异无统计学意义（P>0.05）、NO含量显著下降（P<0.01）。对SOD、MDA、NO含量的影响，健膝丸组与抗骨增生胶囊组比较，差异无统计学意义（P>0.05）。结论：通过对实验各组SD大鼠血清中SOD、MDA、NO水平的检测，结果显示，在膝骨关节炎模型SD大鼠血清中, SOD活性下降，MDA及NO含量升高，由此可知氧自由基等因子在骨关节炎的发病及软骨细胞的破坏进程中起到重要作用。健膝丸对血清MDA的含量影响不明显，但可显著提高SOD活性、清除体内过量的氧自由基，同时抑制了NO的过度产生、降低其生物效应，改善关节微循环、保护软骨细胞或促使受损细胞的修复，从而起到延缓关节软骨退变的作用。%Objective:To probe action mechanism of jianxi pill on preventing knee osteoarthritis by observing its effects on superoxide dismutase(SOD),nitric oxide(NO)and malondialdehyde(MDA) of serum in rats with knee osteoarthritis to provide the scientiifc theory for its clinical practice and development and to further research the pathogenesis of osteoarthritis.Methods:40 SD female rats were randomly and equally divided into four groups: the normal group,the model control group,the jianxi pill group and the kanggu zengsheng capsule group.Except for the normal group,the other three groups were modeled by improved Hulth's technique.After operation,the rats were continuously injected with penicillin(200,000 U) for three days. One week later,the rats were forced to act for half an hour daily for eight weeks.In the meantime,all rats were given corresponding drugs by intragastric administration.According to the humanity and animal body surface area,the equivalent dose was worked out.The normal group and the model control group were continuously given 0.9% physiological saline for eight weeks.Eight weeks later,Rats' abdominal venous blood was extracted and centrifuged for blood serum.According to speciifcations,the content of SOD,MDA and NO were worked out with 754 spectrophotometer.Results:The assaying of SOD、MDA and NO in blood serum showed that compared with the normal control group,the activity of SOD in the model control groups decreased signiifcantly(P<0.01) and the content of MDA and NO increased signiifcantly(P<0.01).Compared with the normal group,the activity of SOD in the jianxi pill group had no obvious statistical difference(P>0.05) and the contents of MDA and NO had statistical difference(P<0.05).Compared with the model control group,the activity of SOD in the jianxi pill group increased signiifcantly(P<0.01) and the difference of the content of MDA had no obvious statistical difference(P>0.05),the content of NO in the group decreased significantly(P<0.01).The difference of the contents of SOD,MDA and NO between the jianxi pill group and the kanggu zengsheng capsule group had no statistical signiifcance(P>0.05).Conclusion:The result showed by measuring the contents of SOD,MDA and NO in rats' blood serum that the activity of SOD decreased and the contents of MDA and NO increased,by which we knew that the oxygen free radical played an important role in the onset of osteoarthritis and destruction of cartilage.Although jianxi pill had no obvious effect on the content of MDA,it could signiifcantly promote the activity of SOD and clear the oxygen free radical.In the meantime,it inhibited over generation of NO,decreasing its biological effect,improving joint's microcirculation,and protecting chondrocyte or promoting the restore of damaged cells,so to postpone the degeneration of articular cartilage.
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