通过2015年11月29日~12月3日一次实时霾、冷空气天气过程与模拟本次冷空气(完全排除了霾的影响)天气过程及PM2.5对动脉粥样硬化大鼠影响的对比实验研究,探讨霾与冷空气共同作用对心血管疾病影响的机理.将24只雄性健康大鼠随机分为4组,每组6只,并制作成动脉粥样硬化模型大鼠,分别为霾与冷空气天气影响组(自然组)、模拟冷空气影响组(模拟组)、PM2.5影响组(PM2.5组)和对照组4组.分别采用三种形式进行影响暴露,一是根据河北省气象台制作的天气和空气质量预报,在2015年11月29日~12月3日进行霾和冷空气天气过程实时对自然组的影响暴露;二是应用TEM1880气象环境模拟箱模拟2015年11月29日~12月3日的冷空天气过程(排除了霾)对模拟组的影响暴露;三是将2015年11月27日~12月3日采集的PM2.5颗粒,制作悬浮溶液,给予PM2.5组气管滴入PM2.5悬浮溶液的影响暴露.检测各组心率、血压和肛温等生理指标,并腹腔主动脉采血和摘取心尖进行匀浆,测量各组动脉粥样硬化大鼠氧化因子(T-SOD、GSH)、炎性因子(IL-6、hs-CRP、TNF-α、sICAM-1)、心血管危险因素(TC、TG、HDL-c、LDL-c、cTnT、Mb)、血管收缩因子(ET-1、NE、EPI、AngⅡ)及反应血栓形成的因子(D-dimer、PAI-1)的生化指标的含量.结果表明动脉粥样硬化大鼠受霾与冷空气的共同影响,各生理、生化指标均比冷空气或PM2.5单独影响变化显著,霾与冷空气具有交互作用,冷空气加剧了霾对冠状动脉及心脏的毒性,使心血管疾病明显加重,甚至导致心肌梗死急性发作猝死,初步探索了霾与冷空气的共同作用对心血管疾病影响的机理.%Through the November 29,2015-December 3 a real-time haze,cold weather process and the simula-tion process of the cold weather(completely ruled out the influence of haze)and PM2.5 effect on atherosclerosis rats contrast experiment research,To discuss Haze and cold air joint action mechanism of the effect on cardiovascu-lar disease.24 healthy male rats were randomly divided into four groups, each group of 6,and made into athero-sclerosis model rats,respectively,haze and the cold weather group(natural group), simulation of cold air influ-ence group(simulated group), PM2.5 impact group(PM2.5 group)and control group in four groups.Using three kinds of forms to affect exposure respectively,First is made according to the hebei province meteorological ob-servatory weather and air quality forecast,on November 29-December 3,2015 for haze and cold weather process in real time to expose the influence of natural group,Simulation case simulation application TEM 1880 meteorological environment on November 29, 2015-December 3 empty cold weather process of simulating the effect of group, Three is to November 27,2015-December 3 collection of PM2.5 particles,suspended solution,give PM2.5 group of trachea drip into the exposed to the influence of PM 2.5 suspension solution.Test each physiological indexes such as heart rate,blood pressure and anus temperature,and abdominal aortic blood and gather the apex homogenate, measuring between groups atherosclerosis rats oxidation factor(T-SOD,GSH),inflammatory factors(IL-6,hs-CRP, TNF-α,sICAM-1)and cardiovascular risk factors(ET-1,NE,EPI,AngⅡ), vasoconstriction factor and reaction of thrombosis factor(D-dimer,PAI-1)of biochemical indexes of content.Results show that atherosclerosis rats by haze and cold air from the combined impact of all the physiological and biochemical indexes than the cold air or PM 2.5 significantly affect change alone, haze interact with cold air, cold air exacerbated the haze of the coronary artery and heart toxicity,aggravate cardiovascular disease significantly,even lead to sudden death of acute myocardial in-farction,preliminary explored the haze and cold air joint action of the mechanism of the effect on cardiovascular dis -ease.
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