双歧杆菌是人和动物肠道中一种重要的益生菌,具有许多生理和保健功能.实验使用厌氧培养箱培养筛选获得双歧杆菌;经鉴定为革兰氏阳性菌(G+).选用海藻酸钠作为成膜材料、羧甲基甲壳素作为保护剂、CaCl2作为固化液,通过挤压法将双歧杆菌包埋在了微胶囊里;将得到的微胶囊分别置于胃液、肠液中并测定其溶解情况,得到了制备微胶囊的配比和最适浓度.使用2.14%海藻酸钠、1.79%羧甲基甲壳素制备双歧杆菌微胶囊,能够得到最好的包埋效果,菌体包埋率可达66.81%;此结果可为双歧杆菌微胶囊的高效生产提供一个参考.%Bifidobacterium is an important probiotics in intestines of human and animals, which plays crucial roles in a lot of physiological and healthy function. Bifidobacterium bacteria samples were cultivated and screened in anaerobic incubator. Selected Bifidobacterium showed bluish violet of Gram-positive bacteria(G +) color under microscope with Gram staining method. The optimal ratio of involved materials was explored for Bifidobacteria mi-crocapsules which could go through gastric juice and release in intestine. An extrusion method was used to prepare Bifidobacteria microcapsules, which involved sodium alginate as film-forming material, carboxymethyl chitin as a protective agent and CaCl2as curing liquid. Optimal ratio of related materials was obtained in preparing microcap-sules by determining dissolutions of immersing resulted microcapsule in gastric juice and intestinal juice respective-ly. The embedding rate reached 66.81% when using 2.14% sodium alginate and 1.79% carboxymethyl chitin in the preparation of Bifidobacteria microcapsules. It can be a useful reference to produce Bifidobacteria microcapsules with high efficiency.
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