致病疫霉(Phytophthora infestans)A2交配型菌株P7723不易产生孢子囊.为了获得大量的孢子囊,试验研究比较了5种不同培养基及7种不同诱导方法对致病疫霉孢子囊产量的影响.研究结果表明:5种培养基中只有燕麦培养基(OMA)和大豆培养基(BA)能产生孢子囊,分别为(106±6.29)、(273±6.86)个/cm2,其余培养基均未产生孢子囊.在7种诱导孢子囊产生的方法中,40 ℃处理、菌丝丛水培处理(10% V8)、皮氏溶液处理和土壤浸出液处理4种方法能诱导原本不产生孢子囊的3种培养基产生孢子囊;其中,40℃处理的诱导效果最好.此外,40 ℃处理也使原本产生孢子囊的OMA、BA培养基上的孢子囊量显著增加.在所有培养基的不同处理中,40 ℃处理的CA培养基上菌株产生的孢子囊量最多,可达到(767±7.98)个/cm2.试验为研究致病疫霉菌生长发育过程中的基因表达分析及遗传转化奠定了基础.%The A2 mating type strain P7723 of Phytophthora infestans is not easy to produce sporangia.In order to get a large number of sporangia,effects of 5 kinds of different media and 7 kinds of different inducing methods on sporangia of P.infestans were compared.The results showed that the strain growing only on oat cultivation base (OMA) and soybean medium (BA) could produce sporangia,respectively (106±6.29) and (273±6.86) cm2,while the strain growing on the rest of the media could not produce the sporangia.Among the seven methods of inducing sporangia production,only 40 ℃ treatment,10% V8 treatment,Petri treatment and soil leaching treatment could induce the production of sporangia in P.infestans growing on the media without inducing effects and 40 ℃ treatment was the best inducing method.In addition,40 ℃ treatment also makes the sporangia of P.infestans growing on the OMA and BA culture medium increased significantly.The sporangium amount of the strain growing on the CA medium with 40 ℃ treatment was the most,reaching (767±7.98) cm2.The foundation for genetic transformation and gene expression analysis of P.infestans at different development stages.
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