[Objective] The objective of the experiment is to explore self-incompatibility (SI) molecular mechanism of apricot at proteome level. [Method] Different proteomics of style at the stage of the small bud, big bud, 24 h after self-pollination (ASP) and 24 h after cross-pollination (ACP) (‘Xinshiji’בBadanshui’) in the SI apricot ‘Xinshiji’ by two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS). [Result] About 1500 protein points were detected, and 66 different proteins were expressed at four different development stage of style in ‘Xinshiji’. Self and non-self pollen had triggered different effects on style at proteomic level. There was one protein expressed special in cross-pollinated style, and four up-expressed and twenty-nine down-expressed in cross-pollinated style, respectively, compared with self-pollinated style. Thirteen protein spots were identified unambiguously. [Conclusion] Different reactions on style at proteomic level had been obviously triggered in ‘Xinshiji’ by selfand non-selfpollen.%[目的]从蛋白质组水平探索杏树自交不亲和的分子遗传机制.[方法]采用双向差异凝胶电泳和质谱检测技术,对自交不亲和杏品种'新世纪'在小蕾期、大蕾期、自花授粉后24 h及异花授粉('新世纪'×'巴旦水杏')后24 h的花柱进行差异性蛋白质组学研究.[结果]在'新世纪'杏的小蕾期、大蕾期、自花授粉后24 h、异花授粉24 h后的花柱中共检测到约1 500个蛋白点,其中有66个差异表达的蛋白点.自交不亲和杏柱头对自体与异体花粉感应在蛋白质组水平有差异,其中异花授粉后花柱中特异表达的蛋白点1个,异花授粉后表达丰度明显升高的蛋白点4个,表达丰度明显降低的蛋白点29个;质谱分析只有13个蛋白质点得到归属鉴定.[结论]明确'新世纪'杏花花柱对自体与异体花粉感应在蛋白质组水平上有差异.
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