[Objective] The objective of the paper is to study the roles of pathogen-originated hydrogen peroxide (H2O2) in the interaction between plant and pathogen. [ Method ] Localization of H2O2 accumulation was investigated in the non-host interaction between rice and Xanthomonas campestris pv. phaseoli (Xcp) by using histochernical analysis under electron microscopy. [Result]The lack of Alkyl hydroperoxide reductase subunit C led to compensatory increase in the activities of H2O2 scavenging, and always resulted in significant decrease of endogenous H2O2 accumulation in ahpC mutant strain compared with the wild-type strain of Xcp,which has impact on the mean level of H2O2 accumulation in this interaction during the interaction with plant. [ Conclusion] The results suggest that bacterial pathogen is a potential source for the H2O2 accumulation in the interaction between rice and Xcp. In addition, the H2O2 produced by bacteria may be an important cause for plant cell injury undergoing the HR during non-host resistance.%[目的]探讨互作体系中病原菌来源过氧化氢的作用.[方法]采用组织化学法通过透射电子显微镜技术观察水稻与油菜黄单胞菌菜豆致病变种互作体系中的过氧化氢积累的定位.[结果]突变体菌株细胞中烷基过氧化物还原酶亚基C的缺失导致胞内其余过氧化氢清除酶活性的净嗜性显著上升,使胞内内源过氧化氢积累水平显著下降,并引起病原菌与水稻互作体系中的过氧化氢积累水平发生显著变化.[结论]病原细菌很可能是互作体系中过氧化氢的一个重要来源.病原细菌产生的过氧化氢很可能在非寄主互作中造成细菌周围植物细胞的损伤,对植物细胞具有潜在的毒性.
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