[目的]研究丛枝菌根真菌(arbuscular mucorrhiz fungi,AMF)对低温胁迫下黄瓜(Cucumis sativusL.)幼苗生长和抗氧化酶活性等生理指标的影响.[方法]以黄瓜品种‘津春2号'为试材,利用人工气候箱进行低温处理(昼/夜,15/10℃),研究低温胁迫下接种AMF对黄瓜幼苗生长、电解质渗透率、根系活力和叶绿素、丙二醛(MDA)、可溶性蛋白质含量及过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响.[结果]与低温胁迫的未接菌对照相比,接种AMF可以使低温胁迫下黄瓜幼苗的株高增长量、茎粗增长量、地上部和地下部鲜质量的增长量、地上部和地下部干质量的增长量均显著提高,可溶性蛋白含量增加10.24%,根系活力提高178.32%,POD、SOD、CAT活性分别提高57.63%、6.72%和35%,MDA含量和电解质渗透率分别降低11.05%和16.08%.[结论]接种AMF可通过促进低温胁迫下黄瓜幼苗叶片可溶性蛋白的大量积累和抗氧化酶活性的提高,来降低膜脂过氧化水平,从而增强黄瓜幼苗对低温胁迫的适应性.%[Objective] The aim of this experiment was to study the effects ofarbuscular mycorrhiz fungi(AMF) on the growth,antioxidant enzyme activities and other physiological indices in cucumber (Cucumis sativus L.) seedlings. [Method] Cucumber cultivar ‘Jinchun 2’ was subjected to low temperature treatment of 15/10℃ ( day / night ) in the artificial climate chamber. The effects of AMF on the growth and the changes in electrolyte leakage rate, root activity, chlorophyll, soluble protein and malondialdehyde (MDA) contents, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities under low temperature stress in the seedling leaves were investigated. [Result] Under low temperature stress, the increments of height, stem diameter, fresh weight and dry weight of root or shoot were suppressed. Compared with the control treatment, AMF-inoculation treatment could significantly promote the increment of height, stem diameter, fresh weight, dry weight, chlorophyll contents by 59.9％-81.7％, soluble protein content by 10.24％, root activity by 178.32％, and increased SOD, POD, CAT activities by 57.63％,6.72％ and 35％, while reduced MDA contents and electrolyte leakage by 11.05％ and 16.08％ in cucumber seedlings leaves under low temperature stress. [Conclusion] AMF-inoculation could promote the accumulation of proline, raise the activities of antioxidant enzymes, decrease the membrane lipid peroxidation and improve the adaptability of cucumber seedlings under low temperature stress.
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