• 主题
高级搜索  >
历史搜索 清空历史
首页> 中文期刊> 《林业科学》 >干旱环境下接种根际促生细菌对核桃苗光合特性的影响

干旱环境下接种根际促生细菌对核桃苗光合特性的影响

         
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

[Objective]Plant growth-promoting rhizobacteria ( PGPR ) are rhizosphere inhabitants that can promote plant growth and suppress diseases. The study of PGPR effect on photosynthetic characteristics will provide scientific basis for application potential under drought stress and technical support for afforestation and vegetation ecological restoration in arid environments. [Method]A pot experiment was conducted in order to evaluate the effects of PGPR on the photosynthetic characteristics under drought conditions. A dominant species of PGPR with the ability to produce cytokinins,namely Bacillus cereus L90,was selected to use in this study. Walnut ( Juglans regia ) seedlings were inoculated with B. cereus L90 and then subjected to moderate soil drought stress. The net photosynthesis rate ( Pn ) , stomatal conductance (gs),intercellular CO2 concentration (Ci) and chlorophyll (Chl) content of the walnut seedlings were measured with a LI-6400 portable photosynthesis system and a portable chlorophyll meter. [Result]The results indicated that drought stress and B. cereus L90 inoculation had significant effects on the Pn,Gs,Ci and Chl content of walnut leaves. Compared with the control,the Pn and Gs under drought stress ( DS) were significantly decreased by 21. 78% and 29. 47%. However,the decreases with inoculation of B. cereus L90 were only 11. 62% and 23. 84%, respectively. Therefore,B. cereus L90 inoculation was able to relieve negative effects of DS on Gs and Pn. The relieving effect of B. cereus L90 inoculation on DS increased along with the prolonged period of stress after the initial stage of drought. The Ci of walnut leaves in DR treatments decreased significantly at the initial stage,and then continuously elevated,whereas the Gs continuously declined. The result suggested that the reductions in net photosynthetic rate resulted from both stomatal and non-stomatal limitations. In B. cereus L90 treatment,Ci and Gs decreased continuously,indicating that the Pn reductions mainly resulted from stomatal limitations. The Chl contents of leaves decreased with drought stress. In the late period of drought stress,the chlorophyll content of walnut seedlings in DR treatment significantly decreased 9. 22%,compared with the control. However,the chlorophyll content with inoculation of B. cereus L90 was 9. 49% higher than that of the DS. B. cereus L90 inoculation could significantly decrease the decomposition of chlorophyll content under drought stress. In re-watering after DS,the Pn,Gs and Chl content were still lower than the control,by 9. 46%,10. 29%and 5. 86%,and the Ci was higher than the control by 8. 72%. However,re-watering the drought-stressed seedlings inoculated with B. subtilis increased Pn,Gs and Chl contents to the control’s level,and the contents were higher than the DR seedlings by 7. 01%,12. 15%,5. 81%,respectively. The photosynthetic ability of B. cereus L90 inoculated seedlings was more easily recovered to the level of control after re-watering.[Conclusion]The present study demonstrated that Pn,Gs and Chl content of walnut leaves significantly decreased due to water stress. Cytokinin-producing,B. cereus L90 inoculation under drought conditions could alleviate non-stomatal limitations and interfere with the suppression of net photosynthetic rate. Additionally,L90 is conductive to the photosynthetic ability recovery of walnut leaves after re-watering.%【目的】研究干旱环境下接种根际促生细菌( PGPR)对植物光合特性的影响，为 PGPR 在干旱逆境中的应用提供依据，为干旱地区造林及植被恢复提供技术支撑。【方法】采用盆栽试验，以产细胞分裂素的蜡样芽孢杆菌 L90为供试 PGPR 菌株，以核桃苗为供试植物，利用 LI-6400便携式光合作用仪研究中度干旱胁迫及干旱复水条件下接种 L90对核桃苗叶片光合参数的影响。【结果】干旱胁迫( DR)及干旱环境下接种L90对核桃苗叶片的净光合速率(Pn)、气孔导度(Gs)、胞间 CO2浓度(Ci)及叶绿素(Chl)含量影响显著。整个胁迫期间，同正常浇水处理相比，DR 处理核桃苗叶片的 Pn和 Gs分别下降21.78%和29.47%，而接种 L90处理仅下降11.62%和23.84%，干旱环境下接种 L90可显著提高 Gs，缓解干旱对 Pn的抑制。除干旱胁迫初期外，随着干旱胁迫时间的增加，L90缓解干旱对 Pn的抑制效果逐渐增强。随着干旱胁迫时间的延长，DR 处理核桃苗叶片的 Ci迅速降低后持续升高，而 Gs持续降低，其光合性能同时受气孔因素和非气孔因素的限制；接种 L90后核桃苗叶片的 Ci和 Gs持续降低，其光合性能主要受气孔因素限制。一定强度的干旱能够造成核桃苗叶片中 Chl 的分解或流失，在胁迫末期，同对照处理相比，DR 处理核桃苗叶片的 Chl 含量显著降低9.22%；同 DR 处理相比，L90处理叶片 Chl的含量显著增加9.49%，干旱环境下接种 L90显著抑制了干旱胁迫后期 Chl的降解。恢复供水后，同正常浇水处理相比，DR 处理的 Pn，Gs和 Chl 含量分别降低9.46%，10.29%和5.86%，Ci增加8.72%；同 DR 处理相比，接种 L90处理的 Pn，Gs和 Chl 含量分别提高7.01%，12.15%和5.81%，基本恢复到对照水平。接种 L90有利于核桃苗叶片光合功能的快速恢复。【结论】干旱胁迫下，植物叶片的 Pn，Gs和 Chl含量均有不同程度的降低，而干旱环境下接种产细胞分裂素的蜡样芽孢杆菌 L90能够在一定程度上抑制这种降低，延缓水分胁迫下非气孔因素对植物叶片 Pn的限制，且更有利于恢复供水后叶片光合功能的恢复。

著录项

  • 来源
    《林业科学》 |2015年第7期|84-90|共7页
  • 作者

    刘方春; 马海林; 马丙尧; 杜振宇; 井大炜; 邢尚军;

  • 作者单位

    山东省林业科学研究院 山东省森林植被生态修复工程技术研究中心山东省核桃工程技术研究中心 济南 250014;

    山东省林业科学研究院 山东省森林植被生态修复工程技术研究中心山东省核桃工程技术研究中心 济南 250014;

    山东省林业科学研究院 山东省森林植被生态修复工程技术研究中心山东省核桃工程技术研究中心 济南 250014;

    山东省林业科学研究院 山东省森林植被生态修复工程技术研究中心山东省核桃工程技术研究中心 济南 250014;

    德州学院 德州 253023;

    山东省林业科学研究院 山东省森林植被生态修复工程技术研究中心山东省核桃工程技术研究中心 济南 250014;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 植物生理学;细菌;
  • 关键词

    干旱胁迫; 根际促生细菌; 蜡样芽孢杆菌; 核桃苗; 光合;

相似文献

  • 中文文献
  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号