首先对猴头菌菌株CBI进行培养特性观察,表明菌株能产生较多的厚垣孢子;对其ITS序列进行扩增(GenBank登录号为GU584100),并与猴头菌属5个种的17个不同地域菌株进行基于ITS序列的系统发育分析,结果表明菌株CB1与猴头菌同种其他菌株遗传距离较近并聚类在一起.明确该菌株的分类地位后,对其进行木质素降解酶系统的检测,结果表明猴头菌可产生锰过氧化物酶(MnP)和漆酶(laccase),但不产生木质素过氧化物酶(LiP).MnP和漆酶的酶活性变化是有规律的,Mn2是猴头菌产生MnP的必要因子,而漆酶的产生则不受该条件的制约.在含Mn2+的LNAS培养基中加入木屑为底物的条件下,猴头菌MnP最大酶活性为45.56 U·L-1,漆酶最大酶活性为61.85 U·L-1.%Researches on ligninolytic enzymes of white-rot fungi could lay a foundation for exploring their degrading mechanism and expression and regulation mechanism of genes encoding for them.The study firstly observed and described the cultural characteristic of Hericium erinaceum strain CB1,result showed that CB1could produce some chlamydospores.Then,its ITS sequence was amplified (GenBank accession number:GU584100),and phylogenetic analyses was made based on the ITS sequences for 5 species including 17 different regional strains from Hericium,results showed that H.erinaceum strain CB1 and other strains from H.erinaceum were nearer in genetic distance and clustered together with them.After the classification status of H.erinaceum strain CB1 was fixed,its major ligninolytic enzymes were detected.The results indicated that H.erinaceum could produce MnP and laccase simultaneously,but no LiP excreted.The activities of MnP and laccase are regular.Mn2 + was proved to be the essential factor for H.erinaceum to produce MnP,but not for laccase.The highest MnP and laccase activity were reached when sawdust was added to LNAS culture solution with Mn2+ substrate which were 45.56 U·L-1 and 61.85 U·L-1,respectively.It is the first time to report that the lignin degrading enzymes of H.erinaceum in China.
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