研究- 80℃下松材线虫的冷冻保存方法,比较不同冷冻保护剂对松材线虫低温保存的保护效果,以及解冻后松材线虫的繁殖情况.结果表明:15％甘油对松材线虫保护效果最好,解冻后存活率达36.5％,其次为10％甘油、20％甘油、25％甘油,保护效果最差的是1％二甲基哑砜(DMSO)；解冻后线虫能够正常生长繁殖,冻存3天和冻存30天的线虫存活率和繁殖无显著性差异.%In this study, we tested the cryopreservation of Bursaphelenchus xylophilus at - 80 ℃ , and investigated effects of different cryoprotectants on the survival and propagation. Cryopreservation with 15% ( V/V) glycerol was found as the best protectant for cryopreservation, followed by 10% ( V/V) , 20% ( V/V) and 25% ( V/V) glycerol. The poorest protecting effect was with 1% dimethylsulfoxide. With 15% ( V/V) glycerol, the survival rate reached to 36.5% after defrosting, and the nematodes retained normal propagation. There was no significant difference in the survival and propagation after cryopreservation between 3-days and 30-days cryopreservation which indicated that cryopreservation with 15% (V/V) glycerol was suitable for B. xylophilus at -80℃.
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