采用SRAP分子标记技术,对采自我国云南、广东、广西、江西、福建、湖南、贵州7个省以及越南安沛省的20个白花树天然群体共269个个体的遗传多样性和遗传结构进行研究.8对引物共获得88个条带,多态性条带81个.白花树在物种水平上的多态条带百分率(PPB)为91.0％,Shannon表型多样性指数(Ho)为0.453 6；在群体水平上PPB为49.49％,Ho为0.284 1,表现出高的遗传多样性.AMOVA分析显示,30.40％的变异存在于白花树群体间,69.60％的变异存在于群体内；群体内与群体间的遗传分化均很明显.Mantel检测表明群体间的遗传距离与地理距离存在显著的相关性.%Styrax tonkinensis is a woody species with multiple uses of timber, ornament, medicine and oil. We examined the genetic diversity and genetic structure of 20 populations which included 269 individuals of 5. tonkinensis distributed in Yunnan, Guangdong, Guangxi, Jiangxi, Fujian, Hunan, and Guizhou Provinces of China, and Yen Bai of Vietnam by using SRAP markers. A total of 88 bands were amplified with 8 SRAP primers, of which 81 bands were polymorphic. Percentage of polymorphic bands (PPB) and Shannon's phenotypic diversity index (Ho) were respectively 91. 0% and 0. 453 6 at the species level, 49. 49% and 0. 284 1 at the population level, reflecting a high level of genetic diversity. Analysis of molecular variance ( AMOVA) revealed that the total genetic variation was 30. 40% and 69. 60% among populations and within populations, respectively. There was significant genetic differentiation within population and among populations. The Mantel test showed that the genetic distance was significantly positively correlated with the geographical distance.
展开▼
