A MYB gene, PeMYBF1, was isolated from male inflorescence of Populus euramericana for the first time by a homologous cloning technique. Sequence analysis showed that the gene encoding protein PeMYBF1 contained only two tandem repeats of 53 amino acids in its N-terminal, which was characteristic of R2R3-MYB transcription factor, suggesting PeMYBF1 is a putative member of R2R3-MYB gene family in P. euramericana. Phylogenetic analysis based on the amino acid alignment of PeMYBF1 and other MYB proteins revealed that PeMYBF1 belonged to subgroup 19, in which members have been known to play important roles in inflorescence development. Organ-specific expression analysis revealed that PeMYBF1 was predominantly expressed in both male and female inflorescence. Our results suggested PeMYBF1 would be involved in the regulation of flower development in P. euramericana. Moreover, temporal expression pattern of PeMYBF1 revealed that PeMYBF1 expression was tightly regulated during inflorescence development.%利用同源克隆方法首次从欧美杨雄花序克隆到1个R2R3-MYB基因PeMYBF1.序列分析结果显示:该基因编码的蛋白质具有典型R2R3-MYB转录因子序列特征,即N端含有2个由53个氨基酸组成的MYB结构域,暗示PeMYBF1是1个欧美杨R2R3-MYB转录因子家族的新成员.聚类分析表明:PeMYBF1归属为第19亚组,该亚组成员在花发育过程中发挥重要作用.器官特异性表达模式分析结果显示:PeMYBF1特异在雄花序和雌花序中表达,暗示PeMYBF1可能参与欧美杨花发育的调控.进一步的分析结果显示:PeMYBF1在不同发育时期花序中的表达水平受到严格调控.
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