Two major anthocyanins of the flower color pigment of Prunus mume ‘ Nanjing Hongxu' were isolated with MeOH-HOAc-H2O ( 10: 1: 9, V/V), and purified by paper chromatography and subsequent column chromatography. Specific chemical reactions, TLC, PC, UV-Vis spectra, HPLC, GC, 1 H NMR, 13 C NMR and FAB MS analyses indicate that the anthocyanins are cyanidin 3-O-( 6"-O-α-rhamnopyranosyl-β-glucopyranoside ) and cyanidin 3-O-( 6"-O-galloyl-3"-O-β-glucopyranosyl-β-glucopyranoside) respectively. In addition to contributing to the mauve flower color, the anthocyanins may improve the ability of P. mume ‘Nanjing Hongxu’ to survive in cold.%梅花‘南京红须'花色色素的2种主要花色苷可用甲醇-乙酸-水(10:1:9)提取,再用纸层析和柱层析纯化.特征性颜色反应、薄层层析、纸层析、紫外-可见光谱、高效液相色谱、气相色谱、核磁共振谱和快原子轰击质谱分析表明:2种花色苷分别是花青素-3-氧-(6"-氧-α-吡喃型鼠李糖基-β-吡喃型葡萄糖)苷和花青素-3-氧-(6"-氧-没食子酰-3"-氧-β-吡喃型葡萄糖基-β-吡喃型葡萄糖)苷.花青苷除决定‘南京红须’的紫红花色外,还可能强化‘南京红须’在寒冷环境中的生存能力.
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