Objective:To observe the effect of premature rupture of membranes in neonatal umbilical cord blood mononuclear cell count (Monocyte count,MONO),neutrophil (Polymorphonuclear neutrophil,PMN) con-tent on the predictive value of neonatal infection.Methods:110 cases of full-term premature rupture of fetal mem-branes were treated in our hospital from August 2014 to August 2017 as observation group,and 110 normal newbo-rns were selected as control group.The serum C reactive protein (CRP),interleukin-6 (IL-6),leukocyte count (WBC),calcitonin (PCT) and platelet count (PLT) in the two groups were compared.The pathological diagnosis of placenta,the rate of neonatal infection and the expression of soluble myeloid cell trigger receptor -1 (TREM-1) and neutrophil CD64 (CD64) in the umbilical cord blood were observed.The neonates in the observation group were di-vided into the infection group and the non infection group according to whether the infection occurred.The expres-sion levels of TREM-1 and CD64 MFI on the surface of MONO and PMN were observed in the two groups.Results:The levels of CRP,WBC,PLT,PCT and IL-6 in the observation group were significantly higher than those in the control group (P＜0.05) ;The neonatal infection rate of chorioamnionitis and postpartum 1 weeks (perinatal period) were 34.55% and 22.73% in the observation group,respectively,and 11.82% and 6.36% in the control group,re-spectively.The difference was statistically significant (P＜0.05) ;The ratio of umbilical cord blood MONO,PMN surface trem-1 and CD64 MFI was significantly higher than that in the control group (P＜0.05),and the ratio of MONO and PMN surface trem-1 and CD64 MFI was significantly higher than that in the control group (P＜0.05). Conclusion:Premature rupture of membranes and neonatal infection are closely related to the expression of TREM-1 and CD64 on the surface of PMN and MONO of umbilical cord blood.Strengthening their clinical detection is helpful to guide early prediction of neonatal infection.%目的:观察胎膜早破时新生儿脐血单核细胞计数(MONO)、中性粒细胞(PMN)含量对新生儿感染的预测价值.方法:选择110例足月胎膜早破新生儿为观察组,并选取同期正常新生儿110例为对照组,比较两组血清C反应蛋白(CRP) 、白细胞介素-6(IL-6) 、白细胞计数(WBC) 、降钙素原(PCT) 、血小板计数(PL T)变化,观察胎盘病理诊断结果、新生儿感染率及脐血可溶性髓样细胞触发受体-1(TREM-1)、中性粒细胞CD64(CD64)表达水平.并将观察组新生儿按照是否出现感染分为感染组与未感染组,观察两组MONO 、PMN表面TREM-1 、CD64 MFI表达水平.结果:观察组CRP 、WBC 、PLT 、IL-6 、PCT 水平较对照组显著升高(P＜0.05) ;观察组绒毛膜羊膜炎、产后1周(围产儿期)内新生儿感染率分别为34.55%、22.73%,对照组分别为11.82%、6.36%,差异有统计学意义(P＜0.05) ;观察组脐血MONO 、PMN表面TREM-1 、CD64 MFI比值显著高于对照组(P＜0.05) ,感染组脐血 MONO 、PMN 表面 TREM-1 、CD64 MFI 比值显著高于对照组(P＜ 0. 05).结论:胎膜早破及新生儿感染与脐血PMN 、MONO表面TREM-1 、CD64表达密切相关,强化其临床检测有助于指导早期新生儿感染预测.
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