目的：探讨尿激酶（Urokinase ，UK）对大鼠肺成纤维细胞凋亡的影响。方法：5只SD雄性大鼠气管内灌注博莱霉素造肺纤维化模型，将模型组肺成纤维细胞与不同浓度的尿激酶培养24～96h ，流式细胞术测定细胞凋亡。结果：尿激酶以时间和浓度依赖的方式抑制肺成纤维细胞生长，流式细胞术检测到凋亡小体。结论：尿激酶能抑制肺成纤维细胞增殖，与凋亡有关。%Objective:To study the effect of urokinase on apoptosis of pulmonary fibroblasts in rats . Methods :Five SD male rats were modeled pulmonary fibrosis modelling by endotracheal perfusion bleomycin .Pulmo‐nary fibroblasts in the model group were cultured with urokinase of different concentrations for 24~96 hours .Cell apoptosis was examined by flow cytometry .Results :Urokinase inhibited pulmonary fibroblasts in time and concen‐tration dependent manners .Apoptosis bodies were found by flow cytometry .Conclusion:Urokinase can inhibit the proliferation of fibroblast ,which is related to cell apoptosis .
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