Objective:To observe the inhibitory effects on the invasion and metastasis of Multiple Myelo‐ma RPMI8226 cells line by small hairpin RNA targeting PIK3R1 in vitro .Methods :The recombinant plasmid vector expression vector which contained PIK3Rl shRNA was transfected into RPMI8226 cells .Real‐time PCR and Western blot were used to detect the expression of P1K3Rl .ELESA was used to measure the change in the MMP‐2 /MM P‐9 expression .The invasion ability of the tumor cells was examined by Transwell tests .Resuits :pGenesil‐1 .1‐PIK3R1 mediated shRNA targeting PIK3R1 dramatically down‐regulated their expression in RPMI8226 cells .MMP‐2 and MM P‐9 were downregulated ,and TIMP‐2 was upregulated .The extracellular levels of MMP‐2 and MMP‐9 were de‐creased .Transwell showed that the number of cells invading through the matrigel in control ,nonsense sequence . And pGenesil‐1 ,1‐PIK3R1 transfection groups were 115 .5 ± 3 .9 ,112 .8 ± 6 .0 ,and 73 .7 ± 4 .0 respectively .Conclu‐sion :ShRNA targeting PIK3R1 down‐regulated significantly their expression in a sequence‐specific manner ,and in‐hibited the invasion of Multiple Myeloma RPMI8226l cells in vitro .%目的：观察应用 RNA 干扰（RNAi）技术敲低 PIK3R1表达后在体外对人类多发性骨髓瘤细胞系 RPMI8226细胞侵袭的抑制作用。方法：将重组质粒表达载体 pGenesil‐1．1‐PIK3R1转染至 RPMI8226细胞。 Realtime PCR 和 Western blot 分别检测转染前后目的基因 mRNA 和蛋白的表达水平，酶联免疫吸附试验（ELISA）检测细胞外基质金属蛋白酶（MMP）‐2、MM P‐9的浓度变化。 Transwell 实验评价肿瘤细胞侵袭能力的变化。 结果：pGenesil‐1．1‐PIK3R1可以有效抑制PIK3R1 mRNA 及蛋白的表达，MM P‐2、MM P‐9表达下调，基质金属蛋白酶组织抑制因子（TIMP）‐2表达上调，ELISA 证实细胞外 MM9‐2、MMP‐9浓度在治疗组明显减低。 Tramwell 穿过细胞数：对照组（115．5±3．9）和无义序列组（112．8±6．0），pGenesil‐1．1‐PIK3R1转染组（73．7±4．0）。结论：靶向 PIK3RI 的 RNAi 技术可以序列特异性地抑制 PI3KR1表达，在体外明显抑制RPMI8226细胞的侵袭能力。
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