Objective:To observe the expression of tumor associated antigen Eps8(epidermal growth fac‐tor receptor pathway substrate 8 ,Eps8)in ovarian cancer cell strain A2780 ,CP70 ,SKOV3 and SKOV3/DDP ,screen out the cell strain CP70 which is at a high level in Eps8’s expression ,observe the changes of Eps8 protein in Cell CP70 under the action of Cisplatin(DDP) ,and discuss the role of Eps8 in chemotherapy resistance in ovarian cancer . Methods :Detected the Eps8’s expression level of mRNA in ovarian cancer cell strain by real‐time Fluorescence Quantitative PCR ,and using Western blot method to detect Eps8 protein expression level in order to screen out the high‐expression Cell CP70 .And then the Western Blot was used to detect the changes in Eps8’s expression in Cell CP70 after treatment of 24 and 48 hours with different concentration of DDP .Result :Eps8 protein’s expression was significantly elevated with the increase of drug concentration and effect time .Conclusions :Eps8 protein’s expression will increase after the cisplatin treatment on Cell CP70 ,which is also dependent on dose and time of DDP treatment . Besides ,the DDP could induce the increase of Eps8’s expression in Cell CP70 .So we can conclude that Eps8 can play an important role in the formation mechanism of cisplatin resistance in ovarian cancer .%目的：观察肿瘤相关抗原Eps8在卵巢癌细胞株A2780、CP70、SKOV3、SKOV3／DDP中的表达情况，筛查出Eps8高表达细胞株CP70。观察顺铂（DDP）作用下CP70细胞中Eps8在蛋白水平表达的变化，探讨Eps8在卵巢癌化疗耐药中的作用。方法：用实时荧光定量PCR法检测Eps8在卵巢癌细胞株中mRNA的表达水平，用Westernblot法检测Eps8蛋白表达水平，筛选出高表达株CP70。用不同浓度DDP作用于CP70细胞24h、48h，用Westernblot法检测Eps8在蛋白水平的变化。结果：随着药物浓度的增加及作用时间的延长，Eps8在蛋白水平的表达明显升高。结论：顺铂处理CP70细胞后，Eps8的蛋白表达量升高，且和DDP作用的时间及剂量有依赖性，即DDP可诱导CP70细胞中Eps8表达的上调。因此推测，Eps8在卵巢癌顺铂耐药形成机制中发挥重要作用。
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