目的:探讨SD雄鼠去势后的细胞凋亡及γ-氨基丁酸(GABA的表达改变,以此来推测雄激素及去势对雄鼠的致痫的影响.方法:取健康SD雄性大鼠44只,随机分为4组:空白对照和生理盐水对照各10只；正常致痫组和去势致痫组各12只.采用戊四氮亚惊厥剂量(35mg/kg)腹腔注射造模,观察记录大鼠潜伏期及发作时间等.点燃后采用心内灌注固定取脑,对脑组织标本行HE染色及GABA、腺嘌呤核苷酸移位酶-1(ANT1)免疫组化染色,整理数据进行统计学分析.结果:①HE染色显示:空白对照及盐水对照组大鼠海马各区细胞排列整齐,边缘清晰,染色均匀,核仁清晰可见,形态正常.②致痫组海马区域神经细胞排列紊乱,胞浆嗜依红染色,体积缩小,核固缩,核膜皱缩,呈现为三角形或不规则性,部分空泡变,去势组受损神经元数目较非去势组略轻.③海马区GABA免疫组化结果显示:空白对照与生理盐水组各区阳性细胞数无显著性差异,致痫组GABA阳性细胞数明显增多,且去势组增加较非去势组相比明显减轻(P＜0.05).④致痫大鼠海马区ANT1阴性表达.结论:致痫大鼠海马区ANT1阴性表达,癫痫细胞凋亡与ANT1无明确相关性.非去势SD大鼠海马区GABA表达较去势大鼠表达增多,与行为学表现一致.%Objective: The effect of the orchiectomy on the change of ANT] ,GABA expression in hipp-ocamal of pentylenetetrazole induced kindling rats was studyed,in ordered to specul- ate on rats for neutered the influence of epilepsy. Methods: Forty-four healthy male SD rats were randomly divieded into 4 groups: black contrast group(10 rats), saline contrast group (10 rats), epilepsy model group (12 rats), Orchiectomized model group (12rats). The model groups use the sub-eclampsia dosage(35mg/kg)of Pentetra-zole(PTZ) to inject, the change of ANT1 、GABA expression in hippocamal of pentylenete-trazole were observed and the data were statistically analyzed. Results: HE stain :Cells of the hippocampus in the black contrast group and the saline contrast group were normal microscopically. In the epilepsy model group and orchiectomized model group apprared dark and shrunken, with pyknntic nucleus and irregular dispeared chromatin clumps. The ruslts apperad the orchiectomized model group had less acidophilic cells than the epilepsy model group. The observed wim immunohistochemical stain: The nmubers of GABA immunological reacted positive cells were similar in the black contrast group and the saline contrast group, however, it were singnificantly increased in other two groups. The nmubers of GABA immunological reacted positive cells were vere significalltly decreased in epilepsy model groups compared with orchiectomized model groups. 4. The nmubers of ANT1 immunological reacted positive cells had no change in hippocamal of pentylenetetrazole induced kindling rats. Conclusion:The nmubers of ANT1 immunological reacted positive cells had no change in hippocamal of pentylenetetrazole induced kindling rats. 4. Orchiectomized model groups had more espress of GABA in hippocamal, which Consistent with behavior performance.
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