Objective:To study the effects of Calcitonin on apoptosis of articular cartilage and the changes of NO in rabbits with knee osteoarthritis. Methods:36 rabbits were randomly divided into three groups: normal group (group A) , model group (group B)and CT treatment group (group C). There were 12 rabbits in each group. The rabbits in group B and C were made into knee osteoarthritis by Hulth's method. The rabbits in group A weren't dealed by special process, except for incision of skin and articular cavity. The rabbits in group C were interfered by hypodermic injection CT everyday(5U/ kg). The other rabbits were interfered by normal saline. The pathomorphism and apoptosis of articular cartilage, the changes of NO in serum and synovial fluid were observed. Results-.The scores of JP and Mankin in group B were significantly higher than those in group A, articular cartilage injury was more serious, meanwhile, the apoptotic percent of articular cartilage and the content of NO in serum and synovial fluid increased too. Compared with group B, the scores of JP and Man-kin in group C were significantly decreased, the articular cartilage injury reduced evidently, the apoptotic percent of articular cartilage and the content of NO in serum and synovial fluid decreased significantly. Conclusion :CT may reduce articular cartilage injury of KOA by downregulating of NO.%目的:探讨降钙素对兔膝骨性关节炎关节软骨细胞凋亡及NO含量的影响.方法:将36只健康大耳白兔随机分为空白对照组(A组)、模型组(B组)和CT治疗组(C组),B、C两组采用改良Hulth法复制膝骨性关节炎模型,A组除切开皮肤和关节腔外,不做其他处理,从术后第6周开始C组每天皮下注射CT 5u/kg,A、B组给与等量生理盐水皮下注射,连续注射20 d后处死动物.观察降钙素对兔膝骨性关节炎关节软骨病理形态、关节软骨细胞凋亡情况及NO含量的影响.结果:与A组相比较,B组JP和Mankin's评分明显升高,关节软骨损伤明显加重,血清及关节液NO含量和软骨细胞凋亡百分数均明显升高；与B组比较,C组JP评分和Mankin's评分明显降低,软骨损伤明显减轻,血清及关节液NO含量和软骨细胞凋亡百分数均明显降低.结论:CT可能通过降低NO含量而减轻KOA的软骨损伤,对KOA起治疗作用.
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